内源性一氧化氮调节大鼠低氧性肺血管结构的重建

Endogenous nitric oxide regulates hypoxic pulmonary vascular remodeling in rats

目的研究内源性一氧化氮对低氧性肺血管结构重建形成的调节作用。方法大鼠随机分为４组：常氧组（ｎ＝８）、低氧组（ｎ＝８）、低氧＋Ｌ精氨酸（ＬＡｒｇ）组（ｎ＝８）、低氧＋Ｎω硝基Ｌ精氨酸甲酯（ＬＮＡＭＥ）组（ｎ＝６）。以光镜观测各组大鼠肺中、小肌型动脉的相对中膜厚度（ＲＭＴ）及相对中膜面积（ＲＭＡ）和三型肺小血管百分比。结果低氧组大鼠肺中、小肌型动脉ＲＭＴ及ＲＭＡ和肺小血管肌型动脉百分比，较常氧组升高（Ｐ均＜０．０１）。低氧＋ＬＡｒｇ组大鼠肺中、小肌型动脉ＲＭＴ及ＲＭＡ和肺小血管肌型动脉百分比，较低氧组降低（Ｐ均＜０．０１）。低氧＋ＬＮＡＭＥ组大鼠肺中、小肌型动脉ＲＭＴ及ＲＭＡ和肺小血管肌型动脉百分比，较低氧组升高（Ｐ均＜０．０１）。结论内源性一氧化氮对低氧性肺血管结构重建的形成有重要的调节作用。

Objective The study aimed to investigate the role of endogenous nitric oxide in the regulation of hypoxic pulmonary vascular remodeling in rats. Methods Thirty rats were randomly divided into four groups: normoxic group ( n =8), hypoxic group ( n =8), hypoxic+L arginine group ( n =8) and hypoxic+N ω L nitro arginine methyl ester (L NAME) group ( n =6). Relative medial thickness (RMT) and relative medial area (RMA) of pulmonary arteries and the percentage of muscularized arteries were observed in the rats of each groups by using a light microscope. Results RMT and RMA of pulmonary arteries and the percentage of muscularized arteries were obviously increased in hypoxic rats as compared with normal controls, respectively ( P <0.01). L arginine significantly reduced RMT and RMA of pulmonary arteries and the percentage of muscularized arteries of chronically hypoxic rats ( P <0.01). L NAME, however, markedly increased RMT and RMA of pulmonary arteries and the percentage of muscularized arteries of chronically hypoxic rats ( P <0.01). Conclusion Endogenous nitric oxide played an important part in the regulation of the development of hypoxic pulmonary vascular remodeling .