老年期痴呆患者血清载脂蛋白E表型分析

Phenotype of serum Apolipoprotein E of patients with senile dementia

目的探讨测定血清载脂蛋白Ｅ（ａｐｏＥ）表型对老年期痴呆患者的意义。方法应用微量血清等电聚焦（ＩＥＦ）和免疫印迹（ＩＢ）法对１０８例老年期痴呆患者（阿尔茨海默病７３例、多发性梗塞性痴呆３５例）和５７名老年健康人的血清ａｐｏＥ进行了分析。结果患者组的血清ａｐｏＥ４／３表型频率（２８．７０％）明显高于老年健康人组（１０．５３％），差异有显著性（Ｐ＜０．０５）；ａｐｏＥ４等位基因（ε４）频率（３６．１１％）与老年健康人组（１４．０４％）间的差异有非常显著性（Ｐ＜０．０１）。结论ａｐｏＥ４等位基因（ε４）或表型可能是易患老年期痴呆的一种危险因素。

Objective To study the relation between apolipoprotein E(apoE) phenotype and senile dementia. Methods Serum apoE phenotype of 108 patients with dementia including 73 patients with Alzheimer′s disease and 35 patients with multi infarct dementia, and 57 senile health subjects was detected using isoelectric focusing (IEF) and immunoblotting (IB) methods. Results The results showed that the serum apoE4/3 phenotype frequency of the patients with senile dementia (28.70%) was higher than that of senile health subjects (10.53%) P <0.05, and statistically significant difference of ε 4 allele frequency was found between patients with senile dementia (36.11%) and senile health subjects (14.04%) P <0.01. Conclusion These data suggested that apoE epsilon 4 allele (ε 4)or phenotype could be regarded as a risk factor for Chinese senile dementia.