藁本中两种活性成分的高效液相色谱法测定

HPLC DETERMINATION OF TWO ACTIVE CONSTITUENTS IN PLANTS OF GENUS LIGUSTICUM

目的：为中药藁本内在质量的评价提供科学依据，建立了藁本中两种活性成分：阿魏酸（ｆｅｒｕｌｉｃａｃｉｄ）、藁本酚（ｌｉｇｕｓｔｉｐｈｅｎｏｌ）的定量分析方法。方法：反相高效液相色谱法，采用７μｍＮｕｃｌｅｏｓｉｌＣ１８键合相柱，流动相为ＭｅＯＨ—Ｈ２Ｏ（４０～６０），以Ｈ３ＰＯ４调至ｐＨ３６，２萘酚为内标，２８０ｎｍ为检测波长。结果：阿魏酸与藁本酚可达基线分离，两者标准品的进样量分别为０２７～４３８μｇ和０５５～８８０μｇ，对样品峰面积与内标峰面积比呈良好线性关系。平均回收率为９７４％～１００９％。用此法对不同产地野生及市售中国藁本及辽藁本的根及根茎进行了分析，测得了阿魏酸及藁本酚的含量，不同产地的藁本药材中此两种活性成分的含量差别较大。结论：此法简便、快速，适用于药材分析。

AIM: To evaluate the quality of Chinese herb Ligusticum , a RP HPLC method was established to separate and determine the two active constituents, ferulic acid and ligustiphenol, in plants of genus Ligusticum . METHODS: ODS column (Nucleosil C 18 , 7 μm) was used. MeOH H 2O (40∶60) adjusted to pH 3 6 by H 3PO 4 was the mobile phase with detection at 280 nm. 2 Naphthol was used as the internal standard. RESULTS: Ferulic acid and ligustiphenol were separated baselinely. The peak area ratios of the two compounds to internal standard were linear when the injection amount were from 0 27 to 4 38 μg and from 0 55 to 8 80 μg, respectively. The average recoveries were 97 3%～100 9%. Quantitive determination of the two active constituents in the rhizoma of Ligusticum from different habitats was investigated using this method. There was great difference in the quantity of the two active constituents in the rhizoma of Ligusticum from different habitats. CONCLUSION: The contents of ferulic acid and ligustiphenol in genuine drugs of Ligusticum were higher than from other habitats. The results showed that the two active constituents were as a scientific basis for evaluating the genuine drugs of Ligusticum .