人工合成三糖抗原在结核病血清诊断中的初步评价

Evaluation of Tb NT P BSA antigen in the serodiagnosis of tuberculosis

目的评价人工合成三糖抗原在结核病血清诊断的应用前景。方法用人工合成的结核杆菌酚糖酯三糖抗原ＴｂＮＴＰＢＳＡ（ｎａｔｕｒａｌｔｒｉｓａｃｃｈａｒｉｄｅｐｈｅｎｙｌｐｒｏｐｉｏｎａｔｅｂｏｖｉｎｅｓｅｒｕｍａｌｂｕｍｉｎ）作抗原，用ＥＬＩＳＡ法检测了４０例结核患者，５２个健康对照的血清标本。结果本方法的特异性、敏感性分别为：检测抗ＴｂＮＴＰＢＳＡＩｇＧ抗体时为９４２％和２００％；检测抗ＴｂＮＴＰＢＳＡＩｇＭ抗体时为７９６％和４５０％。结论该抗原的特异性、敏感性似不理想。

Objective To evaluate of Tb NT P BSA antigen in the diagnosis of tuberculosis.Method Sera from 40 tuberculosis cases and 52 healthy controls were assayed by ELISA using Tb NT P BSA(containing the terminal triglycosyl unit of the M.tuberculosis phenolic glycolipid) as antigen.Results The sensitivity and specificity were 94.2%,20.0% for IgG and 79.6%,45.0% for IgM antibody activity respectively.Conclusion This antigen is not sensitive,that will limit its use in the serodiagnosis of tuberculosis.