丹参提取物F在再灌注损伤中对胃粘膜抗氧化能力影响

Effect of Dan Shen Extract F(DSEF) on Anti Oxidation of Gastric Mucosa during Reperfusion Injury

目的：探讨丹参提取物Ｆ的胃粘膜保护作用与氧自由基的关系。方法：复制失血性休克再灌注损伤模型，３１只大鼠随机分为单纯失血组（ＨＥ，ｎ＝９），生理盐水对照组（ＮＳ，ｎ＝１０）及丹参提取物Ｆ组（ＤＳＥＦ，ｎ＝１２）；ＨＥ组大鼠在失血性休克２０ｍｉｎ后处死，ＮＳ及ＤＳＥＦ组则在休克２０ｍｉｎ后回输全部放出的血液，维持２０ｍｉｎ后处死。其中ＮＳ组静滴ＮＳ００３ｍｌ／ｍｉｎ，ＤＳＥＦ组静滴ＤＳＥＦ１ｇ／１００ｇｗｔ。结果：ＨＥ组损伤指数为０，ＤＳＥＦ组损伤指数明显低于ＮＳ组（Ｐ＜００１），ＤＳＥＦ组的丙二醛（ＭＤＡ）含量明显低于ＮＳ组（Ｐ＜００１），而超氧化物歧化酶（ＳＯＤ）活性明显高于ＮＳ组（Ｐ＜００１）。

Objective: To examine the protective effects of DSEF on gastric mucosa and the relation with oxygen free radicals (OFR). Methods: 31 Wistar rats were randomly divided into group HE, NS and DSEF. The rats in group HE were killed after hemorrhagic shock for 20 minutes. The rats in group NS and DSEF suffering from hemorrhagic shock for 20 minutes were killed after reperfusion for 20 minutes. During whole procedures, the intravenous infusion of normal saline at 0.03 ml/min was given in group NS. DSEF (1g/100g) was infused in group DSEF. Results: The indexes of gastric mucosal lesions (IGMLs) were 0, 23.18% and 4.42% respectively in group HE, NS and DSEF. As compared with 230.56±19.37 nmol/g in group NS, the content of MDA in group DSEF (94.32±11.32 nmol/gw.t) decreased significantly ( P< 0 01). In comparsion with 134.29±13.35 u/g in group NS, the activity of SOD in group DSEF (298.47±20.12 u/g) increased markedly ( P< 0 01). Conclusion: DSEF can effectively relieve gastric mucosal injury caused by OFR during reperfusion.