扇贝多肽对紫外线A诱导HaCaT细胞Fas、c-fos表达的影响

Effect of polypeptide from Chlamys farreri on ultraviolet A-induced Fas and c-fos expression in HaCaT cells

目的观察扇贝多肽(PCF)对紫外线A(UVA)照射后HaCaT角质形成细胞Fas、c-fos表达的影响,以探讨PCF保护UVA致HaCaT细胞损伤的分子机制。方法体外培养的HaCaT角质形成细胞分为5组:对照组、UVA模型组、5.69mmol/LPCF组、2.84mmol/LPCF组、1.42mmol/LPCF组。蛋白质印迹法检测Fas、c-fos蛋白表达;RT-PCR检测FasmRNA表达;荧光定量PCR检测c-fosmRNA表达。结果 UVA照射后模型组Fas、c-fos表达均显著增加(P<0.01),1.42～5.69mmol/L剂量范围内的PCF可抑制UVA引起的HaCaT细胞Fas、c-fosmRNA及蛋白表达(P<0.05),且有剂量依赖性。结论 PCF可通过抑制Fas、c-fos表达而抑制UVA诱导的HaCaT细胞损伤。

Objective To observe the effect of Polypeptide from Chlamys farreri （PCF） on expression levels of Fas and c-fos in HaCaT cells after ultraviolet A （UVA） radiation,and investigate the molecular mechanism of PCF protecting against UVA-induced damage in HaCaT cells.Methods HaCaT cells were divided into five groups： control group,UVA model group,5.69 mmol /L PCF group,2.84 mmol /L PCF group,and 1.42 mmol /L PCF group.Expression levels of Fas and c-fos mRNA was assayed by PCR.Western blot analysis was used to determine the protein levels of Fas and c-fos.Results After UVA exposure,Fas and c-fos expression increased significantly （P〈0.01）.1.42 - 5.69 mmol/L PCF inhibited UVA-induced mRNA and protein expressions of Fas and c-fos in HaCaT cells in dose-dependent manner（P〈0.05）.Conclusion PCF could attenuate UVA-induced Fas and c-fos expression,which may attribute to its protective effect against UVA-induced damage in HaCaT cells.