尼尔雌醇对去卵巢大鼠胫骨作用的研究

Study of Nylestriol Effect on Bone Histomorphometric Parameters in Ovariectomized Rats

目的了解卵巢切除（ＯＶＸ）大鼠胫骨对雌激素治疗的反应。方法将３３只雌性大鼠随机分成３组，每组１１只：ＯＶＸ组、对照组和治疗组。对ＯＶＸ组与治疗组大鼠行ＯＶＸ手术，建立骨质疏松动物模型，治疗组于ＯＶＸ术后１个月予尼尔雌醇喂养，药物喂养３个月后处死，用骨形态计量学方法检测ＯＶＸ大鼠左后肢胫骨干骺端对尼尔雌醇治疗的反应。结果ＯＶＸ组大鼠骨小梁体积、平均骨小梁宽度、平均骨小梁密度和皮质骨平均厚度（骨骺部分），均较对照组显著减少（Ｐ＜０．０５）；四环素标记表面、类骨质表面和组织水平的骨形成速率等，均较对照组显著增加（Ｐ＜０．０５）；矿化沉积速率、类骨质成熟时间和矿化延迟时间，较对照组缩短（Ｐ＜０．０５）。尼尔雌醇治疗３个月可显著增加ＯＶＸ大鼠干骺端骨量（Ｐ＜０．０５），但仍显著低于对照组（Ｐ＜０．０５）；四环素标记表面、类骨质表面和组织水平的骨形成速率均显著低于ＯＶＸ组（Ｐ＜０．０５）。结论尼尔雌醇能降低骨转换，增加骨量。

Objective To investigate the response of trabecular bone of ovariectomized (OVX) rats to nylestriol. Methods Thirty three female Wistar rats were randomly divided into three groups,11 rats in each: nylestriol treated group (E),OVX and the control (C).The OVX rats were used as a model for osteoporosis.After being fed with nylestriol for 3 months,all rats were sacrificed.The effect of nylestriol on bone microarchitecture and dynamics were studied by bone histomorphometry. Results The data suggest that trabecular bone volume,mean trabecular width ,density and cortical thickness were remarkably reduced in OVX group ( P <0.05) while tetracycline labeled surface, osteoid surface and bone turnover rate were increased in comparison with that in C group ( P < 0.05).The bone turnover rate and trabecular bone volume were improved with the treatment of nylestriol for 3 months in the OVX rats( P <0.05). Conclusions Trabecular bone volume and bone turnover rate can be improved by 3 month administrations of nylestriol in osteoporotic rat models.