促性腺激素释放激素类似物那法瑞林对人乳腺癌细胞BCaP-37生长的影响

Inhibitory effects of LHRH analogue nafarelin on the growth of BCaP 37 human breast cancer cells in vitro

目的：观察促性腺激素释放激素（ＬＨＲＨ）类似物那法瑞林对体外培养的人乳腺癌细胞ＢＣａＰ－３７生长的影响。方法：培养的ＢＣａＰ－３７细胞，经下列条件作用４ｄ后进行细胞计数：（１）加入１０－５～１０－９ｍｏｌ／Ｌ的那法瑞林；（２）加入１０－７～１０－１０ｍｏｌ／Ｌ的雌二醇（Ｅ２）；（３）在含１０－８ｍｏｌ／ＬＥ２的条件下，加入１０－６～１０－８ｍｏｌ／Ｌ的那法瑞林或他莫昔芬；（４）加入１０ｎｇ／ｍｌ或５０ｎｇ／ｍｌ的表皮生长因子（ＥＧＦ）；（５）在含１０ｎｇ／ｍｌＥＧＦ的条件下，加入１０－５～１０－７ｍｏｌ／Ｌ的那法瑞林。结果：那法瑞林在１０－５～１０－９ｍｏｌ／Ｌ浓度下对ＢＣａＰ－３７培养细胞的生长无显著抑制作用，在１０－６～１０－８ｍｏｌ／Ｌ浓度下可拮抗１０－８ｍｏｌ／ＬＥ２对ＢＣａＰ－３７细胞的生长促进作用，对１０ｎｇ／ｍｌＥＧＦ抑制ＢＣａＰ－３７细胞生长的作用无显著影响。他莫昔芬拮抗Ｅ２促进ＢＣａＰ－３７生长的作用与那法瑞林相似。结论：那法瑞林对人乳腺癌细胞ＢＣａＰ－３７体外培养生长无显著抑制作用。

Objectve: To study the effects of luteinizing hormone releasing hormone (LHRH) analogue nafarelin, estradiol(E 2) and tamoxifen on the growth of BCaP 37 human breast cancer cell line in vitro. Methods: BCaP 37 human breast cancer cells (2.5×10 4/well) were cultured in DMEM with 5% no estradiol bovine serum. The number of BCaP 37 cell was counted after 96 h culture in following conditions respectively: (1) with nafarelin at 10 -5 ～10 -9 mol/L, (2) with E 2 at 10 -7 ～10 -10 mol/L, (3) with nafarelin or tamoxifen at 10 -6 ～10 -8 mol/L stimulated by 10 -8 mol/L E 2, (4) with epidermal growth factor (EGF) at 10 ng/ml or 50 ng/ml, (5) with nafarelin between 10 -5 mol/L and 10 -7 mol/L influenced by EGF at 10 ng/ml. Results: Nafarelin had no inhibitory effect on growth of BCaP 37 cell cultured at 10 -5 ～10 -9 mol/L . At the concentrations between 10 -6 and 10 -8 mol/L, nafarelin or tamoxifen could antagonize the cell growth stimulated by E 2 (10 -8 mol/L) respectively, and nafarelin had no effect on growth of BCaP 37 cell inhibited by EGF (10 ng/ml). Conclusion: Nafarelin has no inhibitory effect on growth of BCaP 37 human breast cancer cell in vitro, but can antagonize the cell growth stimulated by estradiol.