N-硝基-L-精氨酸对吗啡、二氢埃托啡精神依赖性的逆转作用

Reverse of N G nitro L arginine on the psychical dependence induced by morphine and dihydroetorphine in mice

目的：利用自行建立的条件性位置偏爱模型，探讨Ｎ－硝基－Ｌ－精氨酸（ＮＯ２Ａｒｇ）对阿片类物质诱导的精神依赖性的影响。方法：通过观察吗啡、二氢埃托啡精神依赖的昆明种小鼠，在皮下注射ＮＯ２Ａｒｇ前后于条件性位置偏爱箱偏爱侧停留的时间，判断小鼠精神依赖情况。结果：４ｍｇ／ｋｇＮＯ２Ａｒｇ使吗啡和二氢埃托啡精神依赖小鼠在偏爱侧停留时间分别由给予ＮＯ２Ａｒｇ前的（６．０９±２．０２），（７．９８±０．７２）ｍｉｎ增加到给予后的（９．３１±１．１０），（９．４８±１．２１）ｍｉｎ（Ｐ＜０．０１）。结论：ＮＯ２Ａｒｇ可逆转吗啡和二氢埃托啡所致的条件性位置偏爱，提示ＮＯ在阿片类药物精神依赖性形成中起重要作用。

Objective: To investigate the effect of N G nitro L arginine (NO 2Arg) on psychical dependence induced by opiates on the basis of improved conditioned place preference model in mice. Methods:The time in biased side was recorded before and after 4 mg/kg NO 2Arg administration in morphine and dihydroetorphine psychically dependent mice. Results: 4 mg/kg NO 2Arg could significantly increase the time in biased side in morphine and dihydroetorphine psychically dependent mice. The time in biased side was increased from (6.09±2.02), (7.98±0.72) min to (9.31±1.10), (9.48±1.21) min ( P <0.01). Conclusion: NO 2Arg could reverse the psychical dependence induced by opiates, suggesting that NO plays an important role in the development of opiate psychical dependence.