流动注射快速分析水体中挥发酚及样品保存的研究

Rapid Determination of Volatile Phenols in Waters by Flow Injection Analysis and Study on Water Sample Preservation

采用模块化流动注射分光光度法对环境水中的挥发酚进行测定,并与国家标准方法4-氨基安替比林分光光度法进行对照,测定结果经统计学处理（t=1.94〈t0.05（6）=2.45,P〉0.05）,两种方法无显著性差异;精密度（RSD为1.25%,n=7）、准确度（回收率91%~105%）等各项分析指标符合分析标准。对不同水体中挥发酚保存条件进行了研究,结果表明,水样采集后应立即测试;不能立即测试的水样,加碱（NaOH）保存在低于20℃条件下不超过7天,或加磷酸和硫酸铜保存在4℃条件下不超过3天。

The flow injection analysis（FIA）-spectrophotometric method for the determination of volatile phenols in water sample was studied.The analytical data were compared with those obtained from the standard method of 4-almido antipyrine spectrophotometry.The results showed that there was no statistically significant differences between these two methods（t=1.94t0.05（6）=2.45,P0.05）.The precision（RSD of 1.25%,n=7）,accuracy（recoveries of 91%~105%） and other analytical indexes were in accordance with the analytical criteria.Based on the established FIA-spectrophotometry,the preservation conditions of water samples with volatile phenol were investigated.The results showed that the water samples should be immediately detected after sampling,otherwise the samples should be preserved in alkali condition（pH=13） at temperature of lower than 20℃ for less than 7 days,or preserved in phosporic acid and CuSO4 medium（pH=3.5） at 4℃ for less than 3 days.