氯化镉致人肝癌细胞SMMC-7721线粒体损伤作用

Mitochondrial damage in SMMC-7721 cells induced by cadmium chloride

目的:研究氯化镉对人肝癌细胞SMMC_7721线粒体的损伤作用。方法:分别用5、10、20、30、40μmol/L氯化镉作用体外培养的SMMC_7721细胞24h,实验同时设对照组。采用形态学方法检测其线粒体的结构完整性,用分光光度法检测线粒体钙离子转运能力,采用琼脂糖凝胶电泳法检测线粒体DNA损伤。结果:随着氯化镉的浓度升高,存活细胞数降低,细胞内的线粒体数量减少;氯化镉处理组细胞线粒体钙离子转运能力下降,并可见线粒体DNA损伤条带。结论:氯化镉可以引起人肝癌细胞SMMC_7721线粒体及线粒体DNA损伤。

OBJECTIVE：The mitochondrial damage of SMMC-7721 cells induced by cadmium chloride was studied in vitro.METHODS：The SMMC-7721 cells were treated with cadmium chloride for 24 hours.Morphology was used to assess integrity of mitochondria.Spectrophotography was used to measure the transport ability of Ca^2＋ into mitochondria and agarose gel electrophoresis was used to observe the mitochondrial DNA damage.RESULTS：The mumber of treated cell and mitochondria decreased with increasing cadmium concentration.The transport ability of Ca^2＋ into mitochondria was decreased and the mitochondrial DNA was damaged with increasing concentration.CONCLUSION：Cadmium chloride could damage the mitochondria and its DNA in SMMC-7721 cells.