香加皮水提物对小鼠淋巴细胞免疫调节作用的初步研究

The immunoregulatory activity of cortex periplocae extract on the mice lymphocyts

目的：研究中药香加皮水提取物（cortex periplocae extract,CPE）对小鼠免疫功能的调节作用。方法：无菌分离小鼠淋巴细胞,与不同浓度CPE（250、125、64、32、16、8μg/ml）共同培养一定时间后,采用MTT法测定其对刀豆素A（ConA）诱导的小鼠淋巴细胞转化能力的影响,采用LDH酶释放法测定对NK细胞杀伤活性的影响;采用对肿瘤坏死因子-α（tumor necrosis factor-α,TNF-α）敏感的L929细胞,使用生物法测定CPE对单核细胞产生TNF-α的影响。各实验均设对照组。结果：CPE在8-64μg/ml的浓度范围内可不同程度的提高小鼠体外淋巴细胞转化率和NK细胞杀伤活性,以及促进单核细胞分泌TNF-α,与对照组比较差异均具有统计学意义（P均〈0.05）。结论：一定剂量的CPE可以提高小鼠淋巴细胞的免疫功能,从而发挥抗肿瘤作用。

OBJECTIVE：Study on the immunoregulatory function of cortex periplocae extract（CPE）on mice lymphocytes.METHODS：We separated mice lymphocytes with aseptic manipulation and cultivated them with CPE（concentrations were 250,125,64,32,16,8 μg/ml）,Then the effect of CPE on the proliferative activity of mice lymphocytes induced by ConA was measured by using MTT method.The activity of NK cells was assayed by a released LDH assay.The content of tumor necrosis factor-α（TNF-α）was evaluated by biological method.RESULTS：CPE within the range of 8 to 64 μg/ml could markedly stimulate the mice lymphocyte to proliferate and enhance the activity of NK cells.It stimulated monocytes to produce TNF-α as well.CONCLUSION：CPE showed immunoregulatory activity on mice lymphocytes.This could be used clinically for modulating immune response or for treating tumors and other diseases.