摘要
目的探讨Apg-2对H2O2诱导的Bcr/Abl阳性BaF3-P210细胞损伤的影响。方法以H2O2诱导的pMIGR1空载体感染细胞BaF3-MIGR1和稳定表达Bcr/Abl的BaF3-P210细胞为损伤模型,Western blot和RT-PCR检测H2O2损伤后2组细胞Apg-2表达;建立过表达Apg-2的BaF3-MIGR1细胞(BaF3-MIGR1-Apg2)和BaF3-P210细胞(BaF3-P210-Apg2)H2O2诱导的损伤模型,Western blot检测磷酸化组蛋白(γ-H2AX)的表达,免疫荧光法检测γ-H2AX焦点数。结果 H2O2作用后的BaF3-MIGR1和BaF3-P210细胞的Apg-2蛋白和mRNA水平表达均升高(P<0.05)。H2O2作用BaF3-MIGR1和BaF3-MIGR1-Apg2细胞后其γ-H2AX蛋白表达升高,γ-H2AX焦点数亦增加;BaF3-P210和BaF3-P210-Apg2细胞H2O2损伤后γ-H2AX蛋白表达和焦点数均无明显变化。结论 Apg-2可减少H2O2诱导的BaF3-P210细胞γ-H2AX表达,从而可能降低其细胞损伤。
Objective To study the effect of Apg-2 on H2O2-induced damage to Bcr/Abl positive BaF3-P210 cells. Methods Damage models of BaF3-MIGR1 cells infected with pMIGR1 vector and BaF3- P210 cells stably expressing Ber/Abl were induced by H2O2. Expression of Apg-2 in the two models was detected by Western blotting and RT-PCR. Damage models of BaF3-MIGR1 and BaF3-P210 cells with over-expressed Apg-2 were also induced by H2O2. Expression of phosphorylated histone (γ-H2 AX) and its focal points was detected by Western blotting and immunofluorescence, respectively. Results The expression levels of Apg-2 protein and mRNA in BaF3-MIGR1 and BaF3-P210 cells were increased when Apg-2 was exposed to H2O2 (P 〈 0.05). The expression levels of γ-H2AX and its focal points in BaF3-MIGR1 and BaF3-P210-Apg 2 cells were also increased after treatment with H2O2 ( P 〈 0.05 ) . However, no significant alternation was observed in expression of γ-H2AX in BaF3-P210 and BaF3-P210-Apg2 cells after treatment with H2O2. Conclusion Apg-2 can reduce H2O2 -induced expression of γ-H2AX in BaF3-P210 cells, thus decreasing cell damage.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2010年第15期1621-1624,共4页
Journal of Third Military Medical University
基金
教育部博士学科点专项基金(2005631007)~~