巴马猪胆道盐酸消融对肝功能的影响及病理学变化

Pathological change in liver lobe of Bama pigs after selective bile duct ablation with hydorchloric acid

目的观察以5%盐酸为消融剂,氰基丙烯酸酯为栓塞剂对巴马猪行选择性胆道消融栓塞后靶肝叶病理学改变以及对肝功能的影响。方法 5头巴马猪左外叶胆道置管,造影后行选择性左外叶胆道消融并栓塞术,检测术后动物的肝脏功能的变化、HE染色和Masson胶原染色观察组织病理改变和术后存活情况。结果消融术后动物无死亡。术后3 d血清中ALT、AST、ALP达峰值,其后逐渐恢复正常。术后1周时ALT、AST、ALP与正常无明显异常(P>0.05)。术后1个月,靶肝叶中部及边缘组织胶原染色视野面积百分比及靶肝叶羟脯氨酸含量较正常增加(P<0.05)。结论选择性胆道5%盐酸消融及氰基丙烯酸酯栓塞可以导致巴马猪靶肝叶萎缩纤维化。

Objective To study the pathological changes in liver lobe of Bama pigs after selective bile duet ablation with 5% hydrochloric acid as an as ablation reagent and cyanoacrylate as an embolization reagent. Methods A tube was placed in bile duct in swine left liver lobe and selective bile duet ablation was performed. Liver function, pathologic change in liver tissue and survival time of the animals were observed after operation. Results No death occurred after operation. The ALT, AST and ALP levels reached their peak in 3 d after operation and became normal in 7 d after operation. The collagen-stained area in center and border of target liver lobe was larger than normal （ P 〈 0.05 ）. The level of hydroxyproline was higher in target liver lobe than in normal liver lobe （P 〈 0.05 ）. Gonclusion Selective bile duct ablation with 5% hydrochloric acid and cyanoacrylate can lead to atrophy of target liver lobe in Bama swine.