摘要
目的采用HPLC法对胜红蓟药材进行指纹图谱研究。方法用Diaonsil C_(18)(250mm×4.6mm,5μm)色谱柱,乙腈-0.4%磷酸溶液梯度洗脱,流速为1.0 mL·min^(-1),检测波长为360nm。结果胜红蓟指纹图谱中各成分均有较好分离。结论 HPLC指纹图谱法重现性好,可作为控制胜红蓟药材内在质量的标准。
OBJECTIVE A fingerprint of Ageratum conyzoides L.has been studied by HPLC.METHODS Diaonsil-C18 column was used with mixtures of acetonitrile and 0.4 % phosphoric acid as mobile phase in a gradient mode, The flow rate was 1.0 mL·min^-1, The wavelength was 360 nm. RESULTS According to thechromatographic condition, a good fingerprint of geratum conyzoides L has been described.CONCLUSION The method is repeatable and can be used in quality assessment of Ageratum conyzoides L.
出处
《海峡药学》
2010年第7期48-50,共3页
Strait Pharmaceutical Journal
基金
福州市科技计划项目(2007-z-76)
