细胞间粘附分子-1在局部缺血再灌流脑损伤中作用的研究

The role of ICAM 1 during regional ischemic and reperfusional cerebral injury in rats

目的探讨局部脑缺血再灌流后脑微血管内皮细胞表面表达的ＩＣＡＭ１参与的缺血性炎症反应。方法用免疫组织化学的方法观察大鼠左侧大脑中动脉栓堵２小时再灌流０、３、１２、２４、７２、１２０小时脑微血管内皮细胞ＩＣＡＭ１的表达。结果大鼠左侧大脑中动脉栓堵２小时未见ＩＣＡＭ１的表达，栓堵２小时再灌流３小时病变侧脑组织微血管内皮细胞开始出现ＩＣＡＭ１的表达，１２～２４小时达高峰，并持续至７２小时，非缺血侧脑组织微血管未见ＩＣＡＭ１的表达。结论ＩＣＡＭ１参与了缺血性脑损伤早期的病理过程。提示在缺血早期阻断ＩＣＡＭ１的表达可能有助于减轻缺血性脑损害的程度。

Objective To study the role of intercellular adhesion molecule 1 (ICAM 1) in ischemic reperfusional cerebral injury.Methods By immunohistochemistry alone, The expression of ICAM 1 on cerebral microvascular endothelial cells after occlusion of the left middle cerebral artery for 2 hours and reperfusion for 0、3、12、24、72 and 120 hours were observed.Results There was upregulation of ICAM 1 on microvascular endothelial cells of ischemic cerebral tissue which was peaked and retained at 3、24、 and 72 hr respectively after MCAO and reperfusion.Conclusion ICAM 1 may be involved in the early pathologic process of cerebral ischemia. It is also suggested blockage of ICAM 1 expression during early ischemic period may reduce the cerebral damage.