旁路活化补体对枯否细胞吞噬杀菌功能影响的实验研究

Effects of bypass activated complement on bactericidal capacity of Kuppfer cells

目的：证明一定条件下，旁路活化补体可使ＫＣ吞噬杀菌功能失常。方法：观测定量培养的ＫＣ在ＺＡＨＳ作用下吞噬杀菌及分泌杀菌致炎物质功能的动态水平；观察大鼠肠系膜注射ＺＡＨＳ后肝组织的病理改变；以抗人Ｃ３、Ｃ５血清中和的ＺＡＨＳ进行反证。结果：①ＺＡＨＳ处理组，ＫＣ的ＰＩ和ＩＣＢＡ以及胞内Ｏ－２水平先升后降，胞内ＡＣＰ水平只降无升；ＩＣＢＡ与Ｏ－２和ＡＣＰ的水平均显著相关；各指标至６ｈ降至最低；②阻断组Ｏ－２、ＡＣＰ、ＩＣＢＡ在６ｈ水平保持在正常对照组的９７％、８３％和７９％，显著高于ＺＡＨＳ处理组；③肠系膜静脉注射ＺＡＨＳ１２ｈ后，大鼠肝组织轻度变性，部分ＫＣ线粒体受损，与ＫＣ相邻肝细胞出现凋亡改变，阻断组肝组织无明显病变。结论：ＺＡＨＳ中过量的Ｃ３、Ｃ５碎片持续作用能使ＫＣ吞噬杀菌功能下降，对肝组织结构亦有一定间接破坏作用。

Objective: To determine whether bypass activated complement can directly influence intracellular bactericidal activity (ICBA) of Kupffer cells (KCs). Methods: ① Cultured KCs were challenged to different doses of ZAHS at different time phases and then 4 parameters were measured. ② In vivo, the rats were challenged to ZAHS injection through mesenteric vein. The liver was sampled and examined under LM and EM respectively 12 h later. ③ The same procedures were repeated except that ZAHS had been neutralized by the serum against C3 and C5. Results: ① Phagocytic index (PI), ICBA and intracellular superoxide ions of ZAHS treated KCs rose significantly and then fell. Acid phosphatase (ACP) decreased monotonously decreased. ICBA was significantly correlated to both intracellular superoxide ions and ACP. ② After the 12th injection of ZAHS, the pathological changes in rat livers included albuminous degeneration of most hepatocytes, injured mitochondria of some KCs and apoptosis of few hepatocytes adjacent to KCs. ③ Block test showed that the anti serum treated KCs held 79% ICBA, 83% ACP and 97% intracellular superoxide ions respecting to the normal. No evident pathological changes in the livers of the challenged rats were seen. Conclusion: The fragments of C3 and C5 in ZAHS can directly impair phagocytic and bactericidal function of KCs and indirectly injure the structure of the liver. The mechanism of this might be the great release of bactericidal and inflammatogenic intracellular superoxide ions and ACP.