MEBT/MEBO对血管内皮细胞粗面内质网影响的实验研究

Experimental Study of Effect of MEBT/MEBO on Rough Endoplasmic Reticulum of Vascular Endothelial Cell

目的:本课题旨在进一步探讨烧伤湿性医疗技术(MEBT/MEBO)对皮肤溃疡局部血管内皮细胞粗面内质网结构的影响,以期可以从不同侧面揭示MEBT/MEBO的改善微循环、祛腐生肌、促进创面再生与修复作用的分子生物学机制。方法:将40只SPF级SD雄性大鼠随机分为美宝湿润烧伤膏(MEBO)治疗组和贝复济对照组,每组20只,造模后于次日开始用药,观察用药后治疗组和对照组溃疡创面愈合情况、肉芽组织生长情况,并记录创面愈合时间。在造模后第8天,取两组同时相点创面组织,用电镜观察标本的超微结构,主要观察血管内皮细胞中粗面内质网的结构形态。结果:MEBO组大鼠的皮肤溃疡创面愈合情况明显好于贝复济组,创面愈合时间明显短于贝复济组,有显著性差异(P<0.05)。组织病理学检测MEBO组治疗的创面,造模后第8天标本中血管内皮细胞中粗面内质网结构形态多于贝复济组。结论:MEBO较贝复济能明显的缩短实验性SPF级SD雄性大鼠体表皮肤溃疡模型皮肤慢性难愈合创面的修复愈合时间,具有促进创面愈合,减少瘢痕愈合的作用。其部分愈合机制:促进新生毛细血管的增殖等以加速肉芽组织形成。

Objective： To further explore effect of MEBT/MEBO on rough endoplasmic reticulum of vascular endothelial cell at the ulcer wound site, so as to reveal molecular biological mechanisms of MEBT/MEBO in improving microcireulation, removing necrosis tissue and promoting wound regeneration and repair. Method： 40 SD male rats of SPF grade were divided into MEBO treatment group and bFGF control group randomly and evenly （20 for each group） . After rats were done as the established model, the medication was applied to the rats at the point of the 2nd day in both two groups. The wound healing process, granulation tissue growth and healing time in two groups were observed and recorded. At the point of 8th day after modeling, the wound tissue samples in two groups were taken in the same time points, and then the ultra - structure of samples was observed with electron microscopy. The key point of observation was structure and morphol- ogy of rough endoplasmic reticulum in vascular endothelial cells. Results ： Wound healing of skin ulcers in MEBO group was obviously better than that in bFGF group. The wound healing time in MEBO group was significant shorter than that in bFGF group. There was significant differences （P 〈 0. 05 ） in wound healing time between two groups. Conclusion： Compared with bFGF, MEBO can significantly reduce healing time of skin chronic wounds in the experimental SPF SD male rats skin surface model, it can promote wound healing and reduce scar formation. One of the healing mechanisms may be promoting proliferation of new blood capillary so as to accelerate the formation of granulation tissue.