麦角甾醇酵母基因工程菌株的发酵培养条件

Fermentation conditions of ergosterol yeast engineering strain

目的:探讨麦角甾醇酵母基因工程菌株的发酵培养条件,阐明重组菌株可以玉米水解液为发酵培养基代谢合成麦角甾醇。方法:采用不同的培养基质、氮源、玉米浆含量、无机盐、pH检测工程菌株菌株细胞生物量和麦角甾醇含量。结果:发酵工艺参数为玉米水解液10oBx、玉米浆6.5%、硫酸镁0.1%,pH值6.5,接种量10%,28℃摇床振荡培养16h,酵母工程菌(YDH/pGAD-ScERG1)细胞生物量达14.34g.L-1,麦角甾醇含量可达3.05%,麦角甾醇得率为435.93mg·mL-1。结论:酵母工程菌株(YDH/pGAD-ScERG1)ERG1基因的表达有利麦角甾醇合成。

Objective To explore the fermentation conditions of ergosterol yeast engineering strain and illustrate that the yeast strain（YDH/pGAD-ScERGI）can synthesize ergosterol by using corn hydrolysates as fermentation substrates.Methods The different substrates,nitrogen sources,corn syrup contents,inorganic salts,pH were used to measured the cell biomass of engineered strain and ergosterol content.Results When the fermentation parameters of corn hydrolysates 10o Bx,corn steep 6.5%,magnesium sulfate 0.1%,initial pH of 6.5,inoculum 10%,28℃ shaking cultured 16 h,the yeast（YDH/pGAD-ScERG1）cell biomass reached 14.34 g·L-1,the ergosterol content was up to 3.05%,ergosterol yield was 435.93 mg·L-1.Conclusion The ERG1 gene expression of yeast strains（YDH/pGAD-ScERG1）is conducive to the synthesis of ergosterol.