硫化氢预处理延迟相对大鼠心肌缺血再灌注损伤的保护作用

Protective Effect of Hydrogen Sulfide delayed Preconditioning on Myocardial Ischemia-reperfu- sion Injury in Rats

【目的】探讨硫化氢预处理延迟相对大鼠心肌缺血再灌注（IR）损伤的保护作用。【方法】将30只健康成年Sprague-Dawley雄性大鼠随机分成3组：假手术组（S组）、缺血再灌注组（IR组）和硫化氢组（H组）。S组仅开胸并分离左冠状动脉前降支；IR组行左冠状动脉前降支阻断30rain，再灌注120min；H组予以静脉注射硫氢化钠（NaHS）0．05mg／kg，给药后24h同IR组处理。各组分别于缺血前10min（T1）、缺血30min（T2）、再灌注60min（T3）、120min（T4）四个时点记录血流动力学变化。再灌注结束后测心梗面积。【结果】与IR组比，H组血流动力学改善，心肌梗死面积减少（P〈0．05）。【结论】硫化氢预处理延迟相对大鼠缺血再灌注心肌具有保护作用。

[Objective]To explore the protective effect of hydrogen sulfide delayed preconditioning on myocardial ischemia-reperfusion injury in rats. [Methods] Thirty healthy Sprague-Dawley male rats were randomly divided into sham operation group（group S）, ischemia-reperfusion group （group IR） and hydrogen sulfide group（group H）. Group S only underwent thoracic surgery and their ramus descendens anterior coronariae sinistrae were separated. Group IR underwent the coronary artery occlusion for 30min and then received reperfusion for 120rain. Group H was intravenously injected with NariS 0.5mg/kg for 24h and then was treated as group IS. Hemodynamic changes were measured at 10min before ischemia（T1 ）, 30min after ischemia（T2 ）, 60min after reperfusion（T3 ） and 120min after reperfusion（T4 ）. At the end of the reperfusion, myocardial infarct size was measured. [Results]Compared with group I/R, the hemodynamics was improved and myocardial infarct size was reduced in group H（ P d0.05）. [Conclusion] Hydrogen sulfide delayed preconditioning can protect the myocardial ischemia-reperfusion injury in rats.