bcl-2基因反义核酸对HL-60和K562白血病细胞生存的影响

Effects of Antisense Oligonucleotide of bcl-2 Gene on Survival of HL-60 and K562 Cells

目的：探索ｂｃｌ－２基因反义核酸对白血病细胞生存的影响。方法：主要应用细胞培养和细胞克隆的方法，检测了ｂｃｌ－２基因反义核酸对ＨＬ－６０和Ｋ５６２白血病细胞系生存的影响。结果：ｂｃｌ－２基因反义核酸浓度在２～８μｍｏｌ／Ｌ和６～１２μｍｏｌ／Ｌ分别对ＨＬ－６０和Ｋ５６２细胞的生存有明显的抑制效应，呈剂量－效应关系，二者起效应时间均为第３ｄ；ｂｃｌ－２基因反义核酸浓度为４μｍｏｌ／Ｌ时，对ＨＬ－６０和Ｋ５６２细胞集落形成都有明显抑制效应。结论：ｂｃｌ－２基因反义核酸对白血病细胞生存有明显的影响。

Aim: To investigate the effect of antisense oligonucleotide of bcl-2 gene on survival of leukemic cells.Methods: Cell culture and colony assay were used to examine the effect of antisense oligonucleotide on survival of leukemic cells(HL-60 and K562).Results: There is a significant inhibition of the growth and colony formaion of leukemic cells by day 3 of culture within certain concentration range of bcl-2 gene antisense oligonucleotide. Conclusion: There is a significantly inhibitive effect of antisense oligonucleotide of bcl-2 gene on survival of leukemic cells.