血管性痴呆、P3电位与行为相关的研究

Correlation Between Vascular Dementia P3 Potential and Animal Behavior

目的：判断血管性痴呆（ＶＤ）动物的认知功能，并了解类Ｐ３电位与动物电迷宫指标之间的相关性。方法：应用颅外固定小磁铁、舌下静脉注射四氧化三铁粉的方法，建立大鼠ＶＤ动物模型。并对注射铁粉前后各６个时段的大鼠进行大脑诱发电位类Ｐ３测定和电迷宫检查。结果：发现注射铁粉后各时段大鼠类Ｐ３的Ｐ３Ｌ均比注射前有明显延长（Ｐ＜０．０１），均超过痴呆标准；Ｐ３Ａ则较前明显降低（Ｐ＜０．０１）；各时段错误次数（ＮＥ）亦较前显著增多（Ｐ＜０．０１）；总反应时间（ＴＴＲ）亦较前明显延长（Ｐ＜０．０５），均超过痴呆标准；ＮＥ与Ｐ３Ｌ有正相关性（ｒ＝０．７１，Ｐ＜０．０１）。ＮＥ与Ｐ３Ａ有负相关性（ｒ＝０．６２，Ｐ＜０．０１）。结论：ＶＤ大鼠类Ｐ３Ｌ延长，Ｐ３Ａ降低，ＮＥ增多，ＴＴＲ延长；Ｐ３与行为反应有相关性。

Aim: The present study was undertaken to assess the cognitive function in vascular dementia animal and to know the correlation between the P3 potential and the electric maze test. Methods: Vascular dementia model in rats were made by injecting Fe particles into the sublingual vein, after fixing a magnel on the skull surface. In order to know if it was demented or not and how serious it was, the evoked potential was recorded-P3 like potential in 6 rats and animal electric maze test were checked in five different periods after injection. The p3 latency (P3L), which properly represent the cognitive function in brain, and its amplitude (P3A) were observed. The reaction error numbers (N E) and total reaction time ( T TR ) were recorded in the maze test. Results: Three days later, P3L showed significant prolongation after injection ( P <0.01) and was longer than that of the demented standard. P3A was decreased significantly ( P <0.01). The error number ( N E) in all periods were obviously incseased both P3A and N E were exceeded those of their demented standards. N E positively correlated with P3L ( r =0.71, P <0.01) and negatively correlated with P3A ( r =-0.62, P <0.01). After injection, the histological results showed that by gross observation in the brain tissue, there were black changes (Fe particles). In the functional and histological examinations the results showed that, the animal model for vaseular dementia was successfully made by this method. Conclusion: In vascular demantia P3L was prolonged. P3A was decreased, N E was increased and T TR was prolonged. And that, P3 potential was correlated with animal behavier.