紫外分光光度法测定牛黄消炎片中胆酸的含量

The Content of Bile Acid in Taurine Niuhuang Xiaoyan Tablets Detected by Ultraviolet Spectrophotometry

目的建立牛黄消炎片中胆酸的含量测定方法。方法本实验采用紫外分光光度法,以硫酸溶液显色,对样品的提取检测波长为385nm。结果牛黄消炎片中胆酸的含量限度为不低于0.51mg/片,胆酸浓度在0.0209～0.1604mg/mL范围内与吸收度呈良好的线性关系(r=0.9999,n=5),平均回收率为98.97%(RSD=0.24%,n=9)。结论该法简便、快速、稳定、可靠,可用于测定牛黄消炎片中胆酸的含量。

Objective A method of determination of bile acid in Niuhuang Xiaoyan Tablets. Methods Bile acid was determined by UV spectrophotometry. The detection wavelength was 385nm. Results The average levels of bile acid in the Niuhuang Xiaoyan Tablets is 0.51mg one tablet. For the bile acid, the linear ranged over0.0209～0.1604mg/mL with r=0.9999, n=5.The average recovery （n=9） was 98.97%（RSD=0.24%）.Conclusion The methods were proved to be simple, precise sensitive and reproducible. It can be used for the quality control of Niuhuang Xiaoyan Tablets.