摘要
本研究以获得的FaChit1基因cDNA序列设计引物,采用染色体步移技术从高羊茅基因组中分离了一段FaChit1基因启动子区域。序列分析结果显示,该启动子长度为935bp,含有保守性元件TATA和CAAT-Box,以及与植物逆境胁迫应答相关的多个顺式作用元件,如W-box、ABRE、MYB及MYC转录因子结合位点等。另外构建了含不同长度FaChit1启动子区域(-935bp、-651bp、-233bp)与gus基因融合植物表达载体,分别命名为pFaChit1P-Ⅰ、pFaChit1P-Ⅱ和pFaChit1P-Ⅲ,为进一步进行该启动子的功能分析奠定基础。
According to the sequence of the FaChit1 gene fragment obtained,we isolated FaChit1 gene promoter region from tall fescue（Festuca arundinacea） genomic DNA using genome walking method.Sequence analysis revealed that the FaChit1 promoter region was 935 bp long and contained both typical TATA and CAAT boxes,as well as several potential cis-acting elements related to plant stress responses,such as W-box,ABRE,MYB and MYC transcription factor binding sites.Furthermore,we have constructed three promoter-gus expression vectors with different promoter deletions（-935 bp,-651 bp,-233 bp） in this research,designated as pFaChit1P-Ⅰ,pFaChit1P-Ⅱ and pFaChit1P-Ⅲ,respectively,which laid the basis for further investigating the function of FaChit1 gene promoter.
出处
《分子植物育种》
CAS
CSCD
2010年第4期725-729,共5页
Molecular Plant Breeding
