摘要
本研究建立了一种农杆菌介导法转化矮化型番茄Micro-Tom的试管内开花结实体系。以MS为基本培养基,附加2.0mg/L6-BA时,卡那霉素抗性再生芽的花芽诱导率为100%,在附加2.0mg/L6-BA和1mg/LGA即可诱导开花结实。经GUS组织化学染色和基因组PCR检测证实得到了转基因植株,本实验证明了该转基因植株在试管内开花结实体系从子叶共培养至转基因果实的获得最快仅需90d,为外源基因功能研究提供了一种快速方法。
We erected an in vitro flowering and fruiting system for the Agrobacterium-mediated genetic transformation of a miniature dwarf tomato Micro-Tom.Flower buds can be induced from kanamycin resistant shoots at 100% efficient on MS medium supplemented with 2.0 mg/L 6-BA.The resistant shoots can flower and bear fruits on MS medium supplemented with 2.0 mg/L 6-BA and 1.0 mg/L GA.In this research we proved these resistant plants to be transgenic plants by using genomic GUS histochemical staining and PCR detection as well as this in vitro flowering and fruiting system only need 90 days fastest from the co-cultivation of cotyledon to the formation of transgenic fruits.This protocol could be an efficient protocol for functional genomics in tomato.
出处
《分子植物育种》
CAS
CSCD
2010年第4期818-821,共4页
Molecular Plant Breeding
基金
国家科技支撑计划项目(2007BAD59B06)
国家转基因新品种培育重大专项(2008ZX08010-003)共同资助
