黄瓜叶色突变体遗传及连锁的分子标记研究

Inheritance of Leaf Color Mutant and Molecular Marker Linked to Leaf Color Gene in Cucumber

利用叶色正常(绿色)与叶色突变(黄色)黄瓜材料为亲本,配制正反交杂交一代(F1)、回交一代(BC1)及F2代分离群体。以此为试材,对黄瓜叶色突变的遗传规律进行研究,同时利用分子标记技术筛选与叶色突变基因紧密连锁的分子标记。对F1、BC1及F2后代中绿叶植株与黄叶植株比例进行统计,结果表明:叶片绿色对黄色为完全显性,叶片黄色由1对隐性基因控制。利用AFLP技术和BSA方法,筛选到1对引物组合在绿叶和黄叶亲本与绿叶和黄叶池间同时表现多态性,绿叶亲本和绿叶池在大约200bp处有1条特异条带,而黄叶亲本和黄叶池无带。F2代验证发现鉴定结果符合率高达100%。

Normal green leaf color and a yellow leaf color mutant were used as parents to created F1, BC1, and F2 populations to study the inheritance of yellow leaf color mutant and the molecular marker linked to the mutant gene. The results showed that the yellow leaf mutant is conferred by a recessive gene, and normal green leaf color is completely dominant to yellow leaf color. A polymorphic AFLP marker was found between the green and yellow leaf bulks. The green leaf parent and the green leaf bulk had a approximately 200 bp fragment, and no fragment was amplified from the yellow leaf parent and the yellow leaf bulk. The marker was confirmed to be 100% accurate by F2 individuals.