端粒酶活性在氯化镧诱导MDCC-MSB1细胞凋亡中的作用

Role of Telomerase Activity in Apoptotic of MDCC-MSB1 Induced by LaCl_3 in Vitro

探讨端粒酶活性在LaCl3诱导MDCC-MSB1细胞凋亡中细胞中的作用。肿瘤细胞常规培养于RPMI1640培养液中,加入终浓度为3 mmol.L-1的LaCl3,继续培养12,24,36,48 h后,应用琼脂糖凝胶电泳和AO/EB双荧光染色检测细胞凋亡,以FITC-（C3TA2）3PNA为荧光探针检测细胞内端粒长度,以TRAP-PCR银染法检测端粒酶活性。LaCl3浓度为3 mmol.L-1,作用0~48 h,细胞的琼脂糖凝胶电泳和AO/EB双荧光染色法均观察到明显的细胞凋亡变化,细胞内端粒长度缩短,端粒酶活性下降,并呈时间-效应关系。LaCl3可通过抑制MDCC-MSB1细胞端粒酶活性而诱导其发生凋亡。

The role of telomerase in apoptosis of MDCC-MSB1 cells induced by LaCl3 in vitro was discussed.The tumor cell in RPMI1640 was cultivated regularly,and then LaCl3（final concentration： 3 mmol·L-1） was added.After 12,24,36 and 48 h incubation,agar gel electrophoresis and AO/EB fluorescent staining were performed to identify the cell apoptosis.The FITC-（C3TA2）3PNA was used as a fluorescent probe to detect the telomere length in the cell,and TRAP-PCR silver staining was used to detect the telomerase activity.The results showed that in 0~48 h culture with 3 mmol·L-1 of LaCl3,significant apoptosis was observed according to agar gel electrophoresis and AO/EB fluorescent staining.The telomere length was shortened and telomerase activity decreased with clear time-effect relationship.The conclusion was that the LaCl3 might induce MDCC-MSB1 cells apoptosis through its inhibiting effects on the telomerase activity.