帕金森病患者IL-2、IL-6、IL-10的检测及临床意义

Changes of IL-2,IL-6 and IL-10 of Parkionson's Disease Patients

目的:检测帕金森病(PD)患者血清及脑脊液(CSF)中IL-2、IL-6和IL-10含量,为深入研究细胞免疫与PD的关系及从炎症角度干预提供实验依据。方法:采用双抗体夹心ELISA法测定PD组血清及CSF中IL-2、IL-6和IL-10的含量,并与对照组(同期体检健康者)比较。结果:PD组血清IL-2、IL-6和IL-10含量均高于对照组(P<0.01,P<0.05);PD组CSF中IL-6含量高于对照组(P<0.05)。新发PD患者、服左旋多巴制剂及服非左旋多巴制剂组PD患者血清及CSF中IL-2、IL-6、IL-10含量与对照组比较,差异均无统计学意义(P>0.05)。PD组血清及CSF中IL-2、IL-6、IL-10含量与年龄、病程、Webster评分、Hoehn-Yahr分级分期、UPDRS-Ⅱ和UPDRS-Ⅲ评分间均无相关性。结论:①PD患者存在血清及CSF中IL-2、IL-6和IL-10水平的改变,PD发病与细胞免疫功能失调有关;②PD细胞免疫状态的改变不能归因于外源性多巴胺的摄入。

Aim： Changes of interlukin-2（IL-2）, IL-6 and IL-10 in blood and cerebrospinal fluid（CSF） were observed in this study. Methods： The concentrations of IL-2, IL-6 and IL-10 in blood and CSF were measured by enzyme linked immunosorbent assay（ELISA）. The results of different groups were also analyzed with comparision and correlated methods. Results： The concentration of IL-2, IL-6 and IL-10 in the serum of PD patients are higher than that in the controlled group with difference（P〈0.01, P〈0.05）; the concentration of IL-6 in CSF is higher than that in the controlled group with difference（P〈0.05）. There is no difference between drug taken and without drug taken for IL-2, IL-6, IL-10 concentration in serum and CSF（P〉0.05）. IL-2, IL-6, IL-10 concentration neither in serum nor in CSF have no correlation with age, course, Webster scale, H-Y grade and UPDRS scale, too. Conclusion： ① The changes of IL-2, IL-6, IL-!0 in blood and in CSF show that PD patients cell immune is imbalance. ②The changes of cell immune are not resulted from dopamine intaken.