抑黄曲霉乳酸菌的筛选及菌种鉴定

The screening and identification of Lactic acid bacteria against Aspergillus flavus

目的筛选具有抑制黄曲霉(Aspergillus flavus)生长的乳酸菌。方法以各地泡菜、实验室自制泡菜、豆浆渣以及新鲜猪肠、鸡肠道内容物为材料,采用牛津杯法筛选所需菌株。对筛选出的菌株进行生理生化及16S rRNA基因序列同源性分析。结果分离得到756株乳酸菌,其中有6株菌株对黄曲霉的生长有明显的抑制作用。结论实验获得的6株产酸菌,3株为植物乳杆菌(Lactobacillus plantarum),2株为消化乳杆菌(L.alimentari-us),1株为亚利桑那乳杆菌(L.arizonenensis)。

Objective To obtain strains of lactic acid bacteria with inhibitory activity against Aspergillus flavus.Method Strains of lactic acid bacteria isolated from many sorts of pickles,bean dregs,chicken and pig intestines were screened for antifungal activity by agar-well diffusion assay.The isolates were identified with physiological and biochemical characteristics and 16S rRNA sequencing.Result Among the 756 strains isolated,6 strains showed strong inhibitory activity against A.flavus.Conclusion The 6 strains obtained were identified as 3 strains of Lactobacillus plantarum,2 strains of L.alimentarius and 1 strain of L.arizonenensis.