摘要
目的探讨不同来源大肠埃希菌毒力因子的分布及其与耐药性的关系。方法对2003-2008年温州医学院附属第一医院的住院患者不同标本中分离的188株大肠埃希菌用PCR方法扩增毒力因子papC、sfa/foc、afa/dra、hly、cnf1、iutA、aer、fimH、kpsMTⅡ、fyuA。采用VITEK-60自动化微生物分析仪检测大肠埃希菌对常用抗菌药物的敏感性。结果毒力因子iutA、fyuA在不同标本来源的大肠埃希菌中的检出率较高,均>50%,毒力因子aer、fimH在不同标本来源的大肠埃希菌中的检出率差异无统计学意义(P<0.05)。氨苄西林的耐药性与毒力因子aer有关(P<0.05),喹诺酮类抗菌药物环丙沙星,左旋氧氟沙星的耐药性与毒力因子iutA有关,头孢唑啉的耐药性与afa/dra有关,庆大霉素的耐药性与aer有关,复方新诺明的耐药性与sfa/foc、iutA、aer有关,头孢他啶的耐药性与sfa/foc、iutA有关,氨苄西林/舒巴坦的耐药性与aer有关(P<0.05)。结论毒力因子在不同来源大肠埃希菌中的分布有差异,某些毒力因子与大肠埃希菌耐药性相关,应引起临床重视。
Objective To understand the distribution of virulence genes of Escherichia coli(E.coli) from different sources and the relationship with the drug resistance.Methods PCR was applied to amplify virulence genes of papC,sfa / foc,afa / dra,hly,cnf1,iutA,aer,fimH,kpsMT Ⅱ and fyuA in 188 E.coli strains isolated from different samples of hospitalized patients in our hospital from 2003 to 2008.VITEK-60 AMS was used to test susceptibility of E.coli to common used antibiotics.Results The detection rate virulence genes iutA and fyuA was more than 50%.The difference on the detection rate of aer and fimH in different samples was statistical significant(P〈0.05).The resistance to ampicillin was related with virulence gene aer(P〈0.05);Additionally,the resistance to quinolone(ciprofloxacin,levofloxacin) was related with iutA(P〈0.05),the resistance to cefazolin was related with afa / dra,the resistance to gentamicin was related with aer,the resistance to cotrimoxazole was related with sfa / foc,iutA,aer,the resistance to ceftazidime was related with sfa/foc,iutA,and the resistance to ampicillin/sulbactam was related with aer(P〈0.05).Conclusion The distribution of virulence genes of E.coli varied by different samples.Some virulence genes were related with drug resistance of E.coli,close attention should paid on it.
出处
《疾病监测》
CAS
2010年第7期542-545,584,共5页
Disease Surveillance
关键词
大肠埃希菌
毒力因子
细菌耐药
Escherichia coli
virulence gene
drug resistance