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荧光定量PCR方法在空肠弯曲菌检测中的应用

Application of real-time PCR assay for Campylobacter jejuni detection
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摘要 空肠弯曲菌是引起人类食源性疾病的主要病原菌之一。快速、特异检测方法的建立对于该病原菌感染的诊断及鉴别诊断具有重要意义。荧光定量PCR方法作为一种快速诊断方法已经广泛应用于空肠弯曲菌的检测及鉴定。本文从荧光定量PCR方法在空肠弯曲菌病原检测中的建立、发展以及应用等方面进行综述。 Campylobacter jejuni is recognized as one of the major causes of human food-borne diseases.Developing a rapid and specific detection method is important for the infection diagnosis and pathogen surveillance.Real-time PCR,as one of the rapid and sensitive methods,has been widely used to detect and identify Campylobacter jejuni.The aim of this review is to introduce and summarize the establishment,development and application of this method in Campylobacter jejuni.
作者 乔博 张茂俊
机构地区 郑州大学公共卫生学院 中国疾病预防控制中心传染病预防控制所
出处 《疾病监测》 CAS 2010年第7期572-576,共5页 Disease Surveillance
基金 科技部科技重大专项课题"传染病检测技术研究-传染病病原体诊断和组合检测技术"(No.2008ZX10004-002)~~
关键词 空肠弯曲菌 荧光定量PCR 检测 Campylobacter jejuni real-time PCR detection
分类号 R440 [医药卫生—诊断学]
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参考文献36

  • 1Blaser MJ. Epidemiologic and clinical features of Campylobacter jejuni infections [ J]. Infect Dis, 1997,176 ( 2 ) : 103 - 105.
  • 2Fukushima H, Katsube K, Hata Y, et al. Rapid separation and concentration of food-borne pathogensin food samples prior to quantification by viable-cell counting and real-time PCR [J ]. Appl Environ Microbiol,2007,73 ( 1 ) :92 - 100.
  • 3Cheng BY,Yuan J,Ke HH,et al. A real-time PCR assay for the detection and qnantitation of Campylobacter jejuni using SYBR green I and the light cycler[J]. Yale J Biol Meal,2004,77(5 - 6) :125 - 132.
  • 4Rudi K, Hoidal HK, Katla T, et al. Direct real-time PCR quantification of Campylobacterjejuni in chicken fecal and cecal samples by integrated cell concentration and DNA purification [ J ]. Appl Environ Microbiol, 2004,70 ( 2 ) :790 - 797.
  • 5Robinson DA. Infective dose of Campylobacter jejuni in milk [ J]. Br Med J, 1981,282 : 1.
  • 6Mahony JO, Hill C. A real-time PCR assay for the detection and quantitation of Mycobacterium avium subsp, paratuberculosis using SYBR green and the light cycler[ J]. J Microbiol Methods, 2002,51:283 - 293.
  • 7Inglis GD, Kalischuk LD. Direct Quantification of Campylobacter jejuni and Campylobacter lanienae in Feces of Cattle by real-time Quantitative PCR [ J ]. Appl Environ Microbiol, 2004,70 ( 4 ) : 2296 - 2306.
  • 8阳成波,蒋原,黄克和,祝长青.基于TaqMan探针的Real-timePCR定量检测空肠弯曲杆菌[J].中国预防兽医学报,2003,25(3):183-187. 被引量：16
  • 9Bonjoch X, Calvo L, Soler M, et al. A new multiplexed Real-time PCR assay to detect Campylobacterjejuni , C. coli , C. lari , and C. upsaliensis [ J ]. Food Anal Method, 2009,18 (4) : 275 - 282.
  • 10Persson S, Olsen KE. Multiplex PCR for identification of Campylobacter coli and Campflobacter jejuni from pure cultures and directly on stool samples [ J]. J Med Microbiol, 2005,54 : 1043 - 1047.

二级参考文献21

  • 1吴润,赵晋军.空肠弯曲菌研究现状分析[J].中国兽医科技,1995,25(7):16-18. 被引量：23
  • 2J 萨姆布鲁克 E F弗里奇 T曼尼阿蒂斯(金冬雁 黎盂枫 译).分子克隆实验指南 第2版[M].北京:科学出版社,..
  • 3McCarthy N, Giesecke J. Incidence of Guillain-Barre syndrotne followig infection with Campylobscter jejuni[J].Am J Epidemiol,2001,153(6): 610-614.
  • 4Jones D M,Sutcliffe E M,Curry A.Recovery of viable but non-culturable Campylobacter jejuni[J]. J Gen Microbiol, 1991, 137: 2477-2482.
  • 5Klein B S,J M Vergeront,M J Blezer, et al. Campylobacter Infection Associated with Raw Milk[J].JAMA,1986,225:361-364..
  • 6Stonet V, L Sicinschi, F Mcgraud, et al. Rapid detection of Campylobacter jejuni and Campylobacter coli isolated from clinical specimens using the polymerase chain reaction[ J]. Eur J Clin Microbiol. Infect Dis, 1995 ,14. 355-360.
  • 7Wegmuller B, Luthy J. Candrian U. Direct polymerase chain reaction detection on Campylobacter jejuni and Campylobacter coli in raw milk and dairy products[J]. Appl Environ Microbiol, 1993,59(12):4378-4383.
  • 8Nachamkin,I.M J Blaser,L S Tompkins,et al.Campylobacter jijuni Current Status and Future Trends[M].American Society for Microbiology,Washington D.C.1992.
  • 9Moore JE, Corcoran D, Oooley JS, et al. Campylobacter. Vet Res, 2005,36(3) :351-382.
  • 10Bang DD,Moller NE, Scheutz F, et al. PCR detection of seven virulence and toxin genes of Campylobacter jejuni and Campylobacter coli isolates from Danish pigs and cattle and cytolethal distending toxin production of the isolates. J Appl Microbiol, 2003,94 : 1003-1014.

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疾病监测
2010年 第7期

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