6个玉米杂交种种子纯度的SSR鉴定

Identification Seed Purity of Six Maize Hybrids by SSR Markers

玉米杂交种纯度是影响玉米产业发展的重要因素之一,建立快速、准确可靠的纯度检测技术是控制杂交种纯度的有效措施。从30对SSR引物中筛选出6对表现为双亲互补型的引物,建立了6份杂交种纯度鉴定的SSR体系。Umc1002、bnlg1112、bnlg238、Umc1153、phi072、bnlg240可分别用于玉米杂交种农大108、浚单20、郑单958、莱农14、莱农15和鲁玉16的纯度检测。双亲互补型等位变异基因频率在0.028～0.072之间,可较准确地检出样品中的自交株和大部分异型株。SSR鉴定玉米杂交种纯度结果略低于幼苗鉴定,但二者差异不显著,相关系数为r=0.8434。本试验建立的SSR技术体系可用于6个玉米杂交种纯度的快速检测。

Purity of hybrids seed is one of the key factor influencing maize industry.To establish rapid and accurate purity detection technique is an effective measure for maize seed purity controlling.Six primers were selected from 30 pairs of SSR,which gave stable and polymorphic amplification profiles.The amplification bands of hybrids were complementary with that of the parents.Umc 1002,bnlg 1112,bnlg 238,Umc 1153,phi 072 and bnlg 240 could detect the purity of Nongda No.108,Xundan No.20,Zhengdan No.958,Lainong No.14,Lainong No.15 and Luyu No.16,respectively.Alleles frequency of the complement bands were relatively low with a range of 0.028-0.072,which ensured to separate self-cross accurately and off-type individuals with hybrid plants.The results obtained by SSR markers were a little less than that of field plot planting testing,but there were no significant difference when statistical analysis was performed.The correlation coefficient was 0.8434.The SSR identification system established in this paper could be used to identify seed purity of six maize hybrid varieties rapidly.