耐甲氧西林金黄葡萄球菌小鼠菌血症感染模型的建立与评估

Establishment and evaluation of a bacteriemia model in mice after infection with an isolated methicillin-resistant Staphylococcus aureus（ MRSA ）

目的 本研究采用临床分离鉴定的ST-239型耐甲氧西林金黄葡萄球菌（MRSA）感染BALB/c小鼠,建立菌血症感染模型.方法 从小鼠的临床症状、生存曲线和主要组织器官的病理学变化进行了时相性监测,并用该模型验证万古霉素对小鼠菌血症的治疗效果.结果 ST-239型MRSA感染的小鼠血液中细菌含量较高,小鼠炎症反应严重并伴有心、肺等多组织器官的损伤;对小鼠使用万古霉素后可显著降低血液中的细菌量,动物存活率明显提高,主要组织器官的病理学症状减轻.结论 该模型的建立将为进一步研究临床分离的MRSA的病原特性、发病机制、治疗方法等提供可靠的实验动物模型.

Objective To established a bacteriemia model of BALB/c mice after infection with a ST-239 methicillin-resistant staphylococcus aureus（MRSA） strain, which was isolated and identified from Shanghai Huashan Hospital. Methods We monitored the clinical signs and gross observations of MRSA-infected mice, and examined the histopathology among different groups. Results This isolated MRSA strain ST-239 can induced a typical bacteriemia in BALB/c mice, including the severe mortality and extensive histopathologic injury. However, higher survival rate and slight inflammatory injury were observed in vancomycin-treated mices. Conclusion The solid results obatined in this model will benefit us to study the pathogenic characteristics and patholgenesis in MRSA-induced bacteriemia, and propeled us to seek a safety cure approaches in the future.