一起甲型H1N1流感疫情病原检测及其HA与NA基因特性研究

Detection on the pandemic（ H1N1 ） 2009 virus of an influenza epidemic situation and study on the characteristic of HA and NA gene

目的 确认引起一起流感暴发疫情的病原,阐明该病原的血凝素基因（HA）和神经氨酸酶基因（NA）的特性.方法 疫情中最早出现流感样症状病例的咽拭子样本用real-time RT-PCR方法检测甲型H1N1流感病毒核酸,采用鸡胚分离法进行病毒培养,选取两病毒分离株进行HA和NA核苷酸序列测定,并进行基因特性分析.结果 此次流感疫情是由甲型H1N1流感病毒引起的,其HA和NA基因均与参比毒株的HA和NA基因高度同源,NA基因没有发生H274Y突变.结论 本研究的甲型H1N1流感病毒分离株为疫苗亲本株和中国分离株的类似株,对神经氨酸酶抑制剂类药物（如达菲）敏感.

Objective To identify the pathogen of an influenza epidemic situation and analyze the genetic characteristic of hemagglutinin（ HA ） gene and neuraminidase（NA） gene of this pathogen. Methods Real-time RT-PCR was used to dectect nucleic acid of the pandemic（ H1N1 ） 2009 virus from oropharyngeal swabs of initial influenza-like illness in epidemic. The viruses were was inoculated and isolated with embryonated eggs. And the HA gene and NA gene were sequenced to analyze their characteristic. Results The influenza epidemic situation was caused by the pandemic（ H1N1 ） 2009 virus. The HA and NA sequences data showed that the virus had the high homology with reference virus, and the NA sequences had not the H274Y mutation. Conclusion In this study, the pandemic（ H1N1 ） 2009 virus were similar with the vaccine-like virus and the isolated virus of China, and sensitive to oseltamivir.