摘要
目的 应用多位点数目可变串联重复序列(VNTR)分析技术,对新疆南疆地区维吾尔族结核病患者结核分枝杆菌临床分离株进行基因分型,探讨其数目VNTR基因型种类及其分布.方法 收集结核分枝杆菌,采用PCR和琼脂糖凝胶电泳技术,结合BioNumerics 5.0软件,对其24个VNTR位点进行结果分析.结果 分离出151株结核分枝杆菌,分为8个基因群151个基因型,其中Ⅵ群为主要基因群,占44.4%,有67个基因型,其次是Ⅷ群(23.2%)和Ⅳ群(20.5%).结论 新疆南疆地区维吾尔族结核病患者的结核分枝杆菌存在明显基因多态性,且存在主要流行菌群.
Objective To investigate the application of the multiple locus variable numbers of tandem repeats(MLVA) in genotype Mycobacterium tuberculosis(MTB) strains isolated from Uygur in south of Xinjiang, and to understand the characteristics of genotype and distribution. Methods One hundred and fifty-one Mycobacterium tuberculosis strains were collected from Uygur in south of Xinjiang which contains three regions, Kashgar, Hotan and Kizilsu kirghiz. Twenty-four tandem repeats loci in the total genome of MTB were analyzed by PCR and agarose gel electrophoresis method. The characteristics on polymorphism of DNA fingerprinting of 151 MTB strains were analyzed with BioNumerics 5.0 software. Results Twenty-four MLVA loci of 151 MTB strains were analyzed respectively. The results showed that there were obvious polymorphisms of VNTRs. The clustering of genotype showed that these strains could be categorized into 8 gene groups( Ⅰ , Ⅱ , Ⅲ ,Ⅳ, Ⅴ ,Ⅵ,Ⅶ ,Ⅷ) and 151 genotypes. Sixty-seven isolates(44.4% ) belonged to group Ⅵ. 23.2% were group Ⅷ including 35 genotypes, 20. 5% were group Ⅳ including 31 genotypes. The group Ⅵ prevailed mostly in the Kashgar. The group Ⅲ prevailed mostly in the HOTAN. Conclusion The results showed there were obvious polymorphisms of VNTRs of Mycobacterium tuberculosis clinical strains preliminarily. Group Ⅵ was the predominant prevalent strain in south of Xinjiang.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2010年第7期660-664,共5页
Chinese Journal of Microbiology and Immunology
基金
基金项目:国家地区科学基金资助项目(30960356)
兵团博士基金(2009JCl5)