芬布芬对诺氟沙星体内过程的影响

Effects of Co-Administrationof Fenbufen on the Pharmacokinetics of Norfloxacin

目的探讨芬布芬对诺氟沙星体内过程的影响。方法①SD大鼠24只,随机均分4组:诺氟沙星10 mg.kg-1+生理盐水组、诺氟沙星10 mg.kg-1+芬布芬10 mg.kg-1组、诺氟沙星10 mg.kg-1+芬布芬20 mg.kg-1、诺氟沙星10mg.kg-1+芬布芬40 mg.kg-1组。静注诺氟沙星后于不同时间颈静脉取血,HPLC测定诺氟沙星浓度,DAS2.0软件计算药动学参数;②昆明种小鼠40只,随机均分4组:诺氟沙星15 mg.kg-1+生理盐水组、诺氟沙星15 mg.kg-1+芬布芬15 mg.kg-1组、诺氟沙星15 mg.kg-1+芬布芬30 mg.kg-1、诺氟沙星15 mg.kg-1+芬布芬60 mg.kg-1组。尾静脉注射诺氟沙星30 min后处死动物,分离脑组织制备脑组织匀浆,HPLC测定诺氟沙星浓度。结果联用不同剂量芬布芬,诺氟沙星的血药浓度和脑药浓度均未发生明显改变(P>0.05)。结论芬布芬对诺氟沙星的体内过程无明显影响。

OBJECTIVE To investigate the effects of co-administrating fenbufen on the pharmacokinetics of norfloxacin.METHODS 24 Sprague-Dawley rats were randomly divided into 4 groups by norfloxacin 10 mg·kg-1＋saline,norfloxacin 10 mg·kg-1＋ fenbufen 10 mg·kg-1,norfloxacin 10 mg·kg-1＋ fenbufen 20 mg·kg-1,norfloxacin 10 mg·kg-1＋ fenbufen 40 mg·kg-1.Blood samples were collected after norfloxacin intravenous injection through vena caudalis.The concentration of norfloxacin was determined by HPLC.Pharmacokinetic parameters were calculated by DAS software V 2.0.On the other hand,40 kunming mice were randomly divided into 4 groups treated by norfloxacin 15 mg·kg-1＋saline,norfloxacin 15 mg·kg-1＋ fenbufen 15 mg·kg-1,norfloxacin 15 mg·kg-1＋ fenbufen 30 mg·kg-1,norfloxacin 15 mg·kg-1＋ fenbufen 60 mg·kg-1.The animals were euthanized 30 min after nofloxacin intravenous injection through vena caudalis.The brain tissue was collected and grinded into homogenate.The concentration of norfloxacin was determined by HPLC.RESULTS Co-administrated with fenbufen of different dose,the norfloxacin concentrations both in plasma and in brain homogenate were not effected significantly.CONCLUSION There are no effect of fenbufen on the pamacokinetics of norfloxacin.