丙戊茶碱对大鼠大脑皮层星形胶质细胞NGF和IL-1β释放的影响

Effect of propentofylline on NGF and IL-1β release from rat cerebral cortical astrocytes

目的 探讨丙戊茶碱对大鼠大脑皮层星形胶质细胞神经生长因子（NGF）和IL-1β释放的影响.方法 出生1～3 d的SD大鼠,体重6～8 g,原代培养大脑皮层星形胶质细胞,传代培养到第4代时以1×10^5个/ml的密度接种到24孔培养板中,随机分为8组,每组6孔：正常对照组（C组）：无血清培养基继续培养;不同浓度丙戊茶碱组（p1～3组）：分别加入含有丙戊茶碱10、100和1000μmol/L的培养基中;LPS组：加入含有LPS 1 μg/ml的培养基中;P1＋LPS组、P2＋LPS组和P3＋LPS组：分别加入含有丙戊茶碱10、100和1000μmol/L和LPS 1μg/ml的培养基中.于孵育1、3 d时检测上清液IL-1β和NGF的浓度,以反映其释放量.结果 与C组比较,P1组、P2组和P3组星形胶质细胞NGF释放量升高,IL-1β释放量降低,LPS组NGF释放量降低,IL-1β释放量升高（P＜0.05）,P1＋LPS组、P2＋LPS组和P3＋LPS组NGF和IL-1β释放量差异无统计学意义（P＞0.05）;P1组、P2组和P3组星形胶质细胞NGF释放量依次升高（P＜0.05）,3组IL-1β释放量比较差异无统计学意义（P＞0.05）;与LPS组比较,P1＋LPS组、P2＋LPS组和P3＋LPS组星形胶质细胞NGF释放量升高,P2＋LPS组和P3＋LPS组IL-1β释放量降低（P＜0.05）.结论 丙戊茶碱可促进大鼠大脑皮质星形胶质细胞释放NGF,抑制其释放IL-1β.

Objective To investigate the effect of propentofylline on nerve growth factor （NGF） and IL-1βrelease from rat cerebral cortical astrocytes. Methods Primary cultured rat astrocytes from SD rats （1-3 d,weighing 6-8 g） after 4 passages were randomly divided into 8 groups （ n = 6 wells each）： group Ⅰ control （group C）; group Ⅱ , Ⅲ, Ⅳ the astrocytes were exposed to propentofylline 10, 100 and 1000 μmol/L respectively （group P1, P2, P3 ）; group Ⅴ the astrocytes were exposed to LPS 1 μg/ml and group Ⅵ, Ⅶ, Ⅷ the astrocytes were exposed to propentofylline 10, 100 and 1000 μmol/L in addition to LPS 1 μg/ml （group P1 ＋ LPS, P2 ＋ LPS,P3 ＋ LPS）. The astrocytes were then incubated for 3 days in all 8 groups. The concentrations of IL-1β and NGF in the supernatant were detected at 1 and 3 days of incubation using ELISA. Results LPS activated astrocytes resulting in decrease in NGF release and increase in IL-1β release. Propentofylline significantly increased NGF release and decreased IL-1β release from astrocytes incubated alone or with LPS by suppressing activation of astrocytes. Conclusion Propentofylline can enhance NGF release and inhibit IL-1β release from rat cerebral cortical astrocytes.