木犀草素对自发性高血压大鼠心肌组织醛糖还原酶活性影响及机制

The Effect and Mechanism of Luteolin on the Activity of Aldose Reductase from Cardiac Muscle of Rats

目的阐明木犀草素对自发性高血压大鼠心肌组织醛糖还原酶(AR)活性的影响,为深入研究AR在高血压心血管重塑中的作用和机制提供基础。方法采用紫外分光光度法测定木犀草素对自发性高血压大鼠心肌AR活性的影响。采用免疫荧光法测定血管紧张素Ⅱ(AngⅡ)的含量。结果木犀草素组心肌AR活性(0.0542±0.0018)和AngⅡ(110.48±3.79)活性均下降(P<0.05),各组AR活性变化趋势与AngⅡ浓度变化成直线相关性(r=0.9686,P=0.04)。结论木犀草素可显著抑制AR活性,其机制可能与抑制AngⅡ有关。

OBJECTIVE To investigate the intervention effect of luteolin on the activity of aldose reductase （AR） in spontaneously hypertensive rat（SHR）, and to provide experimental evidence for study on the effect and mechanism of AR on attenuating hypertension-induced cardiovascular remodeling. METHODS Ultraviolet spectrophotometry was performed to determine the activity of AR from cardiac muscle of SHR. Immumofluorescence method were performed to determine the content of blood plasm active mass of AngⅡ. RESULTS The activities of AR （0.054 2±0.001 8） and Ang Ⅱ （110.48±3.79） from cardiac muscle of SHR that had been induced by intragastric administration of luteolin were significantly lower than by intragastric administration of normal saline, and were higher than normal group（P0.05）. The changed tendency of AR activity were correlated with content of Ang Ⅱ （r=0.968 6, P=0.04）. CONCLUSION Luteolin can inhibit the activity of AR. The mechanism may be related to decreasing content of AngⅡ.