灰飞虱从冷冻病叶获得水稻黑条矮缩病毒方法的研究初报

Preliminary Report on the Transmission of Rice Black-Streaked Dwarf Virus from Frozen Infected Leaves to Rice by Insect Vector Small Brown Planthopper (Laodelphax striatellus)

研究出一种利用灰飞虱从冷冻病叶中获得水稻黑条矮缩病毒(riceblack-streaked dwarf virus,RBSDV)的方法。以-70℃条件下冷冻保存的RBSDV罹病植株叶片对灰飞虱饲毒,随后接种感病水稻品种,结果发现灰飞虱可从冷冻病叶上获得RBSDV,并传播RBSDV,且获毒能力和传毒能力与新鲜病叶没有差异。这表明从冷冻病叶上获得RBSDV的灰飞虱可以应用于品种抗性鉴定,此法可望加快RBSDV抗病品种的选育进程。

In order to preserve virus for identifying resistant rice varieties against rice black-streaked dwarf disease,a simple and reliable method was developed by which virus-free small brown planthopper（SBPH） acquired rice black-streaked dwarf virus（RBSDV） from frozen infected leaves and transmitted the virus to healthy rice plants.RBSDV acquired from infected leaves frozen at-70℃ by SBPH was transmitted to a susceptible rice variety.The experimental result showed that the SBPH could acquire the virus from frozen infected leaves and transmit it to the susceptible rice variety.For the ability of obtaining and transmitting the virus by SBPH,there was no significant difference between frozen infected leaves and fresh infected leaves.The novel method could be applied to the resistance identification of rice varieties to rice black-streaked dwarf disease and it would facilitate the breeding process for rice black-streaked dwarf disease resistance.