温度对水稻抗褐飞虱特性的影响

Effects of Temperature on Resistance of Rice to Brown Planthopper,Nilaparvata lugens

为研究温度对水稻抗褐飞虱特性的影响,于2007年和2008年应用苗期集团筛选法(SSST)在杭州地区的自然条件下测定了抗性水稻品种IR26和IR36对田间褐飞虱的抗性,同时比较了不同温度(22℃、25℃、28℃、31℃和34℃)下IR26和IR36对褐飞虱的抗性、可溶性糖和草酸含量的变化。自然条件下,IR26已完全失去对杭州种群褐飞虱的抗性,而IR36仍具中等抗性。在25℃到34℃的范围内随着温度升高,IR26对褐飞虱抗性减弱,但22℃条件下抗性比25℃更弱。IR36在温度升高的条件下对褐飞虱的抗性总体呈降低趋势,在31℃和34℃下也已完全失去对褐飞虱的抗性。在22～25℃条件下,IR26和IR36的持抗期均在25℃下最长,分别为9d和11d。3个品种稻株中的可溶性糖含量均随温度升高而增加,而草酸含量则随温度升高先增加后下降,在25℃时为最大值。不同温度下及不同水稻品种之间稻株内可溶性糖和草酸的含量均有显著差异。

To investigate effect of temperature on the resistance characteristics of rice varieties with different resistance genes to rice brown planthopper （BPH）,Nilaparvata lugens （Stl）,the resistances of IR26（Bph1） and IR36 （bph2） to Hangzhou population of BPH were monitored in greenhouse during 2007 and 2008 by using the Standard Seedling Screening Techniques （SSST） developed by the International Rice Research Institute （IRRI）.The changes in resistances of IR26 and IR36 to BPH,soluble sugar and oxalic acid contents in 25-day-old rice plants of susceptible variety TN1 and resistant varieties IR26 and IR36 were detected at five temperatures （22℃,25℃,28℃,31℃ and 34℃）.The results showed that IR26 completely lost its resistance both at natural temperatures and at the five tested constant temperatures,IR36 still had medium level resistance under natural temperature.IR26 and IR36 showed a decreased trend in the resistance from 25℃ to 34℃,and they fully lost the resistance at 31℃ and 34℃.The highest resistance duration for both of IR26 and IR36 was recorded at 25℃.The soluble sugar contents in plants of the three tested rice varieties increased with the increasing temperature,and the oxalic acid content decreased at the temperatures higher than 25℃.Two-way ANOVA indicated the significant effects of temperature and rice variety on contents of soluble sugar and oxalic acid in rice plant.