摘要
在纳米生物学效应研究中,纳米颗粒能否自由进入植物体内是一个亟待考证的关键问题。本文尝试采用一定浓度的水溶性纳米量子点溶液培养玉米幼根,然后徒手切片,用荧光显微镜直接观察发光量子点在根内的运输和分布。结果表明,量子点能够自由进入玉米根且在多种类型的细胞内都有分布;尤其是在导管内也观察到量子点,说明这种纳米材料能够穿透极厚的细胞壁输送到胞内。本文也讨论了该实验设计的优缺点。
In the study of nano-biological effect,it is urgent to find evidence for that if nanoparticles can transport into a plant freely.In this paper,maize seedlings are cultured in Hogland＇s neutrient liquid containing water-soluble quantum dot.Five days later,the seedling roots are sliced by hands and watched by fluorescence microscopy in real time.Results show that the quantum dots can transport freely into the maize roots and distribute in various type cells,especially in the vessel cell,indicating the nanoparticles can penetrate through the extreme thick cell wall and transport into the cells.We also discuss the advantages and disadvantages of this experimental design.
出处
《植物生理学通讯》
CAS
CSCD
北大核心
2010年第7期719-723,共5页
Plant Physiology Communications
关键词
量子点
可视化
玉米根
运输
quantum dot
visualization
maize root
transport
