复合富血小板血浆的硼酸盐生物玻璃修复兔实验性骨缺损

Experimental study on bone formation with a novel borosilicate glass combined with platelet-rich plasma

目的 评价复合富血小板血浆（platelet-rich plasma,PRP）的硼酸盐生物玻璃在兔体内的成骨作用.方法 取36只新西兰大白兔建立双侧桡骨1.5 cm骨缺损模型,随机分为4组,每组9只.A组一侧植入双碱硼酸盐（D-Alk-1B）材料,对侧植入D-Alk-1B＋PRP;B组一侧植入D-Alk-1B＋PRP,对侧植入β-磷酸三钙（β-TCP）;C组一侧植入β-TCP,对侧为实验骨缺损对照;D组一侧植入D-Alk-1B,对侧为实验骨缺损对照.术后4、8、12周取材,通过大体观察、X线片、病理组织学、扫描电镜和Micro-CT评价复合PRP的硼酸盐生物玻璃的成骨作用.结果 术后4、8、12周X线成骨评价和组织学评价结果 基本相似.D-Alk-1B、β-TCP及D-Alk-1B＋PRP三组均比实验骨缺损对照组成骨明显（P＜0.05）;D-Alk-1B＋PRP成骨性能最好,与D-Alk-1B、β-TCP比较差异均有统计学意义（P＜0.05）;D-Alk-1B和β-TCP材料成骨性能相似.组织学观察和扫描电镜结果 显示D-Alk-1B的降解速度比β-TCP快,D-Alk-1B＋PRP降解速度最快,降解后材料的多孔结构消失.Micro-CT观察提示D-Alk-1B在桡骨骨缺损中和宿主骨骨性结合比β-TCP材料好.结论 新型硼酸盐生物玻璃材料具有良好的生物活性、组织相容性和可降解性.修复骨缺损的能力和β-TCP材料相似,与PRP复合有协同成骨作用.

Objective To evaluate the osteogenesis of a novel borosilicate glass materials combined with platelet-rich plasma in repairing segmental bone defects. Methods 36 New Zealand white rabbits which bilateral radius were resected into l.5cm bone defect, were divided into 4 groups averagely depending on implanted materials： group A （one side： D-Alk- 1B, another side： D-Alk- 1B ＋PRP）, group B （one side：D-Alk- 1B ＋PRP, another side： β-TCP）; group C （one side： β-TCP, another side： experimental bone defect）,and group D（one side：D-Alk-1 B,another side ：experimental bone defect）. The specimens were examined after 4, 8,12 weeks; the osteogenesis was evaluated through gross observation, X-ray radiograph,histological examination,scanning electron microscope and Micro-CT. Results There were similar results about gross observation,X-ray radiograph ,histological examination. After 4, 8, 12 weeks ,D-Alk-1B materials, β-TCP and D-Alk-1 B ＋ PRP group had better osteogenesis ability than the experimental control group （P 〈0.05）; D-Alk-lB ＋ PRP had the best performance, better than D-Alk-1B and β-TCP （P〈0.05）; D-Alk-1B were similar to β-TCP （P〉0.05）. D-Alk-1 B materials degradated faster than β-TCP materials, and the porous structure of the materials disappeared after degradation. D-Alk-1B materials intergrated with host＇s bone was better than β-TCP materials. Conclusion D-Alk-1B material have good biological activity, histocompatibility and biodegradation and simiar presence of bone formation compared withβ-TCP in the aspect of repairing the segmental bone defect, the combination of PRP and D-Alk-1 B strengthened osteogenesis in vivo.