纳米银抑制副流感病毒的研究

Inhibiting effect of silver nanoparticles on parainfluenza virus

目的:探讨纳米银(NaAg)对副流感病毒PIV3的抑制作用。方法:采用血球凝集试验、MTT分析法、血球吸附试验和鸡胚培养法、细胞病变(CPE)观察、透射电镜观察细胞形态的方法,探讨NaAg对副流感病毒PIV3的抑制作用。结果:NaAg/PIV3组和副流感病毒PIV3对照组血球凝集试验效价分别为1∶256和1∶1024(两者比较,P<0.01),NaAg溶液对副流感病毒PIV3在鸡胚中的增殖有一定的抑制作用,血凝效价均为1∶512,与副流感病毒PIV3对照组(血凝效价1∶1024)相比差异具有显著的统计学意义(P<0.05);NaAg溶液在MDCK细胞上最大无毒浓度为25μg/mL;副流感病毒PIV3在MDCK细胞上半数感染浓度(TCID50)为10-4.5;25μg/mLNaAg溶液能使20TCID50副流感病毒PIV3感染的MDCK细胞存活率由(39.74±3.01)%提高为(81.45±1.20)%(两者比较,P<0.01),使CPE由(++++)的阳性转为(+),并有效抑制了副流感病毒PIV3血球吸附试验,电镜下观察细胞形态良好。结论:NaAg对副流感病毒PIV3具有抑制作用。

Objective To explore the inhibiting effect of silver nanoparticles （NaAg） on parainfluenza virus PIV3. Methods The inhibiting effect of NaAg on parainfluenza virus PIV3 were determined by hemagglutination test, MTT assay, haemocyanin absorption test, cultivation of the chick embryo, cytopathic effect （CPE） assay, and TEM. Results The titer of hemagglutination test was 1 ： 256 in NaAg/PIV3 group and 1 ： 1 024 in control/PIV3 group （P 〈 0.01 ）. NaAg inhibited the proliferation of PIV3 in the chick embryo. There was a significant difference in the hemagglutination titer between NaAg/PIV3 group and control/PIV3 group （1 ： 512 vs. 1 ： 1 024, P 〈 0.05）. The maximal nontoxic concentration （TD0） of NaAg for a MDCK cell was 25 μg/mL and the TCID50 of PIV3 in a MOCK cell was 10^4.5. NaAg of 25 μg/mL increased the survival rate of the MOCK cell infected by 20TCID50 parainfluenza virus PIV3 from（39.74 ±3.01）% to （81.45 ± 1.20）%（P 〈 0.01）,change CPE from（＋＋＋＋） to （＋） and effectively inhabit the blood corpuscle absorption caused by parainfluenza virus PIV3, the cell shape treated by NaAg was good detected by TEM. Conclusions Silver nanoparticles has an inhibiting effect on parainfluenza virus PIV3.