鼻咽癌发展过程中细胞超微结构的体视学研究

Stereologic study on cellular ultrastructure in the developing course of nasopharyngeal carcinoma

目的:探讨鼻咽癌发生发展过程中形态学特点及其变化规律。方法:应用体视学技术,在超微结构水平对人鼻咽部正常假复层纤毛柱状上皮、疑似鼻咽癌最后病理为上皮增生或慢性炎症以及鼻咽癌细胞株(5-8F和CNE-1)的细胞和细胞核的形态进行了定量测试,用于定量分析的体视学参数包括细胞核的周长(Cn)、面积(An)、体积(Vn)、体积密度(Vv)、数密度(Nv)、表面积密度(Sv)、细胞的周长(Cc)、面积(Ac)共8个参数。结果:8个参数按正常鼻咽上皮→慢性炎症上皮或增生上皮→鼻咽癌细胞株的顺序逐渐增大,且数据在统计学上具有显著性差异。结论:本研究提示鼻咽癌病理发展存在一个由正常上皮→异性增生→癌的逐步增进的过程,这一改变具有病理学意义。

Objective To explore the morphological characteristics in the development of nasopharyngeal carcinoma. Methods Stereological techniques were used in the ultrastructural level to determine the morphological characteristics of normal human nasopharyngeal pseudostratified eiliated columnar epithelium, chronic inflammation, and two nasopharyngeal carcinoma cell line （5-8F and CNE-1） and the nucleus. The stereological parameters for quantitative test include the perimeter of the nucleus （Cn）, area （An）, volume （Vn）, volume density （Vv）, numerical density （Nv）, surface area density （Sv）, cell perimeter （Cc）, area （Ac）. Results The 8 parameters were gradually increased in the normal nasopharyngeal epithelium-→chronic inflammation →nasopharyngeal carcinoma epithelial cell line, and there were significantly differences. Conclusions There may be a pathologic course of nasopharyngeal carcinoma development from normal epithelial hyperplasia→atypical hyperplasia →cancer.