固相萃取反相高效液相色谱法测定人血浆中兰索拉唑的浓度

Determination of Lansoprazole in Human Plasma by RP-HPLC with Solid Phase Extraction

目的:建立以固相萃取反相高效液相色谱法测定人血浆中兰索拉唑浓度的方法。方法:避光条件下采用固相萃取法处理血7.6样),,流反速相为高1效.0液mL相·色mi谱n-法1,柱测温定为兰室索温拉,唑检血测药波浓长度为,2色84谱nm柱,为进样Ve量nu为sil2X0BμPL-。C结18,果流:动兰相索为拉乙唑腈血-药水浓(3度2在:681,0用～21%50氨0n水g·调mLpH-1值范围至内线性关系良好(r=0.9999);平均相对回收率为100.93%,日内、日间RSD<10%。结论:本方法灵敏、简便、快速,可用于血浆中兰索拉唑的测定。

OBJECTIVE：To establish RP-HPLC combined with solid phase extraction method to determine the plasma concentration of lansoprazole.METHODS：The sample was extracted by solid phase extraction in darkness.RP-HPLC was applied using Venusil XBP-C18（200 mm×4.6 mm,5 μm） column.The mobile phase consisted of acetonitrile-water（32：68,pH value adjusted to 7.6 with 1% ammonia water） and flow rate was 1.0 mL·min^-1.The detection wavelength was set at 284 nm and column temperature was set at room temperature.RESULTS：The linear range of lansoprazole was 10～2 500 ng·mL^-1（r=0.999 9） with mean recovery of 100.93%.The RSD of intra-day and inter-day were both less than 10%.CONCLUSION：The method is sensitive,convenient and rapid for the determination of lansoprazole concentration in plasma.