固定化青霉素酰化酶生产6-APA的流程设计

CALCULATION ON REACTION PROCESS USING IMMOBILIZED PENICILLIN AMIDASE WITH THE KINETIC EQUATION

针对本组研制的一种新型固定化青霉素酰化酶，以动力学方程为基础，分别进行了批式反应和连续反应的计算．对于批式反应，计算了不同浓度底物的水解过程和各种抑制因子对反应过程的影响；对于连续反应，计算了不同级数下的需酶量、转化率和成本消耗。结果表明，三、四级连续反应是可行的，有工业应用的前景。

Reaction of a novel immobilized penicillin amidase developed in the authors' group was calculated with the kinetic equation. The effect of substrate and products on the enzymatic reaction in batch mode was also analyzed. Although batch reaction is usually adopted in the industrial production of 6-APA, continuous reaction is more attractive. In the case of continuous operation, the consumption of enzyme, conversion and cost with different number of reaction stages were examined. The results suggest that three- or four-stage continuous reaction has greater potential.