摘要
用商业化RPMI1640添加15mgml-BSA将154枚兔原核卵,经体外24h、46h、70h、94h和118h培养,分别有98.7%(152/154)、98.1%(151/154)、92.9%(143/154)、86.4%(133/154)和75.3%(116/154)的胚胎得到进一步发育.56.5%(87/154)经118h培养后发育成胚泡.140h后孵化率达35.7%(55/154).将70枚发育的4-细胞移植到5只受体,3只妊娠(60%)产仔5只.结果表明在RPMI1640中添加BSA,可完成兔受精卵植入前的发育,且发育的4-细胞在植入受体输卵管后,可发育到妊娠期满并产仔.
Total 154 rabbit pronuclear eggs were cultured in commercialized chemical defined medium, RPMI1640 supplemented with 15 mg·ml BSA. After 24 h, 46 h, 70 h,94 h and 118 h cultured in vitro, development rate was 98.7%(152/154), 98.1%(151/154), 92.9%(143/154), 86.4%(133/154) and 75.3%(116/154), respectively. 56.5%(87/154) developed to blastocyst after 118h culture, and 35.7%(55/154) hatched after 140 h.70 (4 cell embryos) were transferred to five recipients, five live youngs were obtained from three recipients. The results suggest the RPMI1640 with BSA is capable of supporting development of rabbit embryos from pronuclear stage to blastocyst. It is feasible to transfer 4 cell embryos into recipient oviducts for development of full term.
出处
《内蒙古大学学报(自然科学版)》
CAS
CSCD
1999年第2期223-226,共4页
Journal of Inner Mongolia University:Natural Science Edition
基金
国家自然科学基金