已知成分培养液中兔原核卵培养到胚泡的研究

Development of Rabbit Embryos from Pronuclear Stage to Blastocyst in a Defined Culture Medium

用商业化ＲＰＭＩ１６４０添加１５ｍｇｍｌ－ＢＳＡ将１５４枚兔原核卵，经体外２４ｈ、４６ｈ、７０ｈ、９４ｈ和１１８ｈ培养，分别有９８．７％（１５２／１５４）、９８．１％（１５１／１５４）、９２．９％（１４３／１５４）、８６．４％（１３３／１５４）和７５．３％（１１６／１５４）的胚胎得到进一步发育．５６．５％（８７／１５４）经１１８ｈ培养后发育成胚泡．１４０ｈ后孵化率达３５．７％（５５／１５４）．将７０枚发育的４－细胞移植到５只受体，３只妊娠（６０％）产仔５只．结果表明在ＲＰＭＩ１６４０中添加ＢＳＡ，可完成兔受精卵植入前的发育，且发育的４－细胞在植入受体输卵管后，可发育到妊娠期满并产仔．

Total 154 rabbit pronuclear eggs were cultured in commercialized chemical defined medium, RPMI1640 supplemented with 15 mg·ml BSA. After 24 h, 46 h, 70 h,94 h and 118 h cultured in vitro, development rate was 98.7%(152/154), 98.1%(151/154), 92.9%(143/154), 86.4%(133/154) and 75.3%(116/154), respectively. 56.5%(87/154) developed to blastocyst after 118h culture, and 35.7%(55/154) hatched after 140 h.70 (4 cell embryos) were transferred to five recipients, five live youngs were obtained from three recipients. The results suggest the RPMI1640 with BSA is capable of supporting development of rabbit embryos from pronuclear stage to blastocyst. It is feasible to transfer 4 cell embryos into recipient oviducts for development of full term.