盐胁迫下小麦新品系89122的抗氧化酶类活性变化的研究

Analyses of Enzymic Antioxidant Defenses in New Wheat Line 89122 under Salt Stress

利用受粉后的花粉管通道将高梁ＤＮＡ导入普通小麦陇春１３号，在其后代中出现了广泛变异，从中选育出已稳定遗传的丰产、抗逆性强的新品系８９１２２．经大田和盐池鉴定，８９１２２的耐盐性明显高于陇春１３号．用ＮａＣｌ（０，０．１，０．１５，０．２，０．２５ｍｏｌ／Ｌ）处理８９１２２和陇春１３号幼苗７ｄ，测定盐胁迫后其幼苗中抗氧化酶类活性的变化．结果发现，转基因小麦８９１２２较其受体陇春１３号能维持较高的ＳＯＤ，ＣＡＴ及ＰＯＤ活性．并且以上几种酶之间能保持很好的平衡和协调表达．表明具有较高的清除活性氧的酶活力与小麦耐盐性紧密相关，可将其作为转基因小麦耐盐筛选的指标之一．

DNA of sorghum ( Sorghum bicolor ) was introduced into 'Longchun No.13' ( Triticum aestivum L.) through the pollen tube pathway after self pollination. A great range of different kinds of variation occurred in the progenies of receptor and a good variation line-89122 with stabilized heredity was obtained. It gave high yield and has been identified stress-tolerance. In this study, the enzymatic antioxidant defenses in seedlings of 89122 and Longchun No.13 were analysized after both were exposed to salt stress. The activities of superoxide dismutase (SOD),catalase(CAT) and peroxidase (POD) were higher in 89122 than in Longchun No.13. These results suggest that higher activities of enzymic antioxidant defenses are associated closely with salt tolerance and they can be adopted as an index in selecting transgenic wheat.