丹参叶片无性系快速繁殖及植株再生研究

Clonic reproduction and plant regeneration from blade of Salvia miltiorrhiza Bunge

不同光质和温度对丹参叶片启动率、愈伤组织和芽形成率均有不同的效应．以绿光诱导芽形成频率最高，达９６％．３０℃条件下芽的诱导频率提高，可达９９％．一般，从丹参叶片接种到植株再生只需３０～４０ｄ；每块０．５ｃｍ２的叶片可产生２０～４０个芽．试管苗移栽后的存活率为９０％～９５％．

There was different effect with various wavelengths of light and temperature on initiation, callus and bud formation frequency of blade in Salvia miltiorrhiza Bunge . Under green light, the frequency of buds induction was the highest, up to 96%. The frequency of buds induction was increased in 30℃, being 99%. In general, it only needed 30～40 d from blade inoculation to plant regeneration. A 0.5 cm 2 blade could produce 20～40 buds. Survival rate of plantlets was up to 90%～95% after they had been transplanted into pots.