异黑成熟颗粒醇提取工艺

Alcohol Extraction Technology of Abnormal Savda Munziq Granular

为筛选异黑成熟颗粒中甘草、地锦草、铁线蕨和香青兰最佳的醇提取工艺，采用单因素试验，以甘草酸含量和醇浸出物量为指标，对影响提取效果的乙醇浓度A、提取时间B、乙醇用量C和提取次数D进行考查，确定提取次数为2次，选择乙醇浓度为55%、65%、75%，乙醇用量为10、12、14倍量，提取时间为1.0、1.5、2.0h。进一步采用L9（34）正交试验设计优选甘草、地锦草、铁线蕨和香青兰醇提取条件，对实验各关键因素变化范围内均衡抽样，使每次试验都具有较强的代表性。结果表明，乙醇浓度对提取效果有显著影响，而提取时间、乙醇用量对提取效果无显著性影响，最佳提取工艺为A2B2C3。鉴于乙醇用量和提取时间对提取效果无显著性影响，为降低成本、节省工时，将B2改为B1，将C3改为C1，由此得提取工艺为A2B1C1。确定药材醇提取条件：65%乙醇10倍量，提取2次，每次1h。由此，经过正交试验法优选的提取工艺设计合理，结果稳定可行，符合大工业生产要求。上述实验结果可为异黑成熟颗粒制备工艺的确定提供实验依据。

To develop the optimal alcohol extraction technology of Gancao, Dijincao, Tiexianjue in the Abnormal Savda Munziq Granula, with the content and the amount of alcohol extractive of glycyrrhizin as index, single factor experiments were carried out. The four factors that influence the extraction result, the quantity and the concentration of alcohol, the extracting time and the frequency, were optimized. The extracting process is as follows： to extract twice with 55%, 65%, 75% alcohol, of amount of 10, 12, 14 times, 1.0, 1.5 and 2.0 hours each time. Then the L9（3^4） orthogonal design is used to find the best alcohol extraction technology of Gancao, Dijincao, Tiexianjue, Xiangqinglan. A balanced sampling is adopted in the range of each key factor to make each experiment highly representative. With the well-distributed nature of these factors, the orthogonal layout can ensure all experiments serve their purpose. The results show that the extracting time and amount do not have marked influence but the concentration of alcohol does. Due to the insignificant influence of the amount of alcohol and the extracting time, the optimum extraction technology A2B2C3 may be replaced by A^BICI, that is, to extract twice with 65% alcohol, of amount of 10 times, 1 hour each time. The extraction method is reasonable, feasible and convenient to be applied to production. The preparation technology for Abnormal Savda Munziq Granular can be similarly obtained.